| IED ID | IndEnz0002011203 |
| Enzyme Type ID | protease011203 |
| Protein Name |
Putative ubiquitin thioesterase L96 EC 3.4.19.12 |
| Gene Name | L96 |
| Organism | Tipula iridescent virus (TIV) (Insect iridescent virus type 1) |
| Taxonomic Lineage | Viruses Varidnaviria Bamfordvirae Nucleocytoviricota Megaviricetes Pimascovirales Iridoviridae Betairidovirinae Iridovirus (small iridescent insect viruses) Invertebrate iridescent virus 1 Tipula iridescent virus (TIV) (Insect iridescent virus type 1) |
| Enzyme Sequence | MCDIDLKTCEKSSKYKKKDIVDLGKKCGVDPYLPNGREKTRQVICTEIVNQYSTNPPSSSSESDDDEDMGKQDQDPICGISEKECNKLDNVNLLALGEYCGVDIYTPEGNLKTSKTICKSVSLKQSSPKAQKSPNKNDLINMDPANLKKLAKKLDIDWKKKDIKELQYLIAKKLNSIKSVSPSRSPSPRRSRSPSPNIQNMKKKELKALAKALGASDKKLDKMDKNQLIEYILSRKKSPSPVRKSRSPSLRQRSRSPGRSRSNSPVRPKYRAADLLGKKVVELKELAKMEGLKKWKDKTPSKMLKQDLVDFLLHVGGGGQLPKTPSPVKPSASPKTRAELALLKVPELKAMALSYGLKNFKDKTPSQLRKNDFIDFIILSTQKSQSPPPSIRSKPRSSSPKYKTKDLVTTDESDGEIVVKKITRKPKSPRRSPPASVRRSRTPSVPKSPSARPRSKSPSVRAEITDDEGETPPSSVRPKSPSVRPKSPSVRPRSKSPSVRPKSPSARPRSKSPSVRSKSPSVRPKSPSVRPKSPSVRPRSKSPSVRPKSPSVRPPPPDPSNSPSITVDPSVTPPSSVKIKKRPELPEYKGGNADRDLEILARRRGYKVIPVKGDGNCLFRAVGKSLRLNQNIKYSHEDLRAQVVTYLTSHKEFLEPYLEYVTESGDTTPQEYAKNVERYIKNISKPGTWGDFICLRVLSEILKVKFNLLILNTRNFQVISNNDTFKPLIPLGFIDDYHYTALTPLYAEPIAVLENETPTPSIAPSIRPPPSIIPSIAPSVRPSIAPSVRPSIVPATPSIVPSLKPSVVPKLTPSIAPKIPPPVFGPVKPLSSTRELLEEADKVHPYVREDISQLARAEEQILVSLGM |
| Enzyme Length | 867 |
| Uniprot Accession Number | P22856 |
| Absorption | |
| Active Site | ACT_SITE 614; /evidence=ECO:0000250|UniProtKB:Q96FW1; ACT_SITE 617; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q5VVQ6; ACT_SITE 738; /evidence=ECO:0000250|UniProtKB:Q5VVQ6 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000250|UniProtKB:Q5VVQ6}; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Hydrolase that can remove conjugated ubiquitin from proteins and may therefore play an important regulatory role at the level of protein turnover by preventing degradation (By similarity). May be involved in TIV genomic DNA packaging in a manner related to the Gag polyproteins of the mammalian viruses. {ECO:0000250, ECO:0000269|PubMed:1701750}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Compositional bias (8); Domain (1); Region (4) |
| Keywords | DNA-binding;Hydrolase;Protease;Repeat;Thiol protease;Ubl conjugation pathway;Viral genome packaging;Viral release from host cell |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 96,012 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |