| IED ID | IndEnz0002011232 |
| Enzyme Type ID | protease011232 |
| Protein Name |
Scytalidopepsin B SLB EC 3.4.23.32 Acid protease B |
| Gene Name | |
| Organism | Scytalidium lignicola (Hyphomycete) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Leotiomycetes Leotiomycetes incertae sedis Scytalidium Scytalidium lignicola (Hyphomycete) |
| Enzyme Sequence | MKFTTAAVLSALVSAEIAFAAPGGNGFARRQARRQARAAGLKASPFRQVNAKEATVESNWGGAILIGSDFDTVSATANVPSASGGSSAAGTAWVGIDGDTCQTAILQTGFDWYGDGTYDAWYEWYPEVSDDFSGITISEGDSIQMSVTATSDTSGSATLENLTTGQKVSKSFSNESSGSLCRTNAEFIIEDFEECNSNGSDCEFVPFASFSPAVEFTDCSVTSDGESVSLDDAQITQVIINNQDVTDCSVSGTTVSCSYV |
| Enzyme Length | 260 |
| Uniprot Accession Number | P15369 |
| Absorption | |
| Active Site | ACT_SITE 190; /note=Proton acceptor; /evidence=ECO:0000269|PubMed:14993599 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins with broad specificity, cleaving 24-Phe-|-Phe-25, but not 15-Leu-|-Tyr-16 and 25-Phe-|-Tyr-26 in the B chain of insulin.; EC=3.4.23.32; |
| DNA Binding | |
| EC Number | 3.4.23.32 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (20); Chain (1); Disulfide bond (3); Helix (1); Propeptide (1); Sequence conflict (4); Signal peptide (1); Site (1); Turn (2) |
| Keywords | 3D-structure;Aspartyl protease;Direct protein sequencing;Disulfide bond;Hydrolase;Protease;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..20; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (4) |
| Cross Reference PDB | 1S2B; 1S2K; 2IFR; 2IFW; |
| Mapped Pubmed ID | 17069854; |
| Motif | |
| Gene Encoded By | |
| Mass | 27,165 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.23.32; |