| IED ID | IndEnz0002011243 |
| Enzyme Type ID | protease011243 |
| Protein Name |
Abasic site processing protein YedK EC 3.4.-.- |
| Gene Name | yedK yedG b1931 JW1916 |
| Organism | Escherichia coli (strain K12) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Escherichia coli (strain K12) |
| Enzyme Sequence | MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKVLLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADGWFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIHDRRPLVLSPEAAREWMRQEISGKEASEIAASGCVPANQFSWHPVSRAVGNVKNQGAELIQPV |
| Enzyme Length | 222 |
| Uniprot Accession Number | P76318 |
| Absorption | |
| Active Site | ACT_SITE 2; /note="Nucleophile"; /evidence="ECO:0000269|PubMed:31235915, ECO:0000269|PubMed:31504793" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.-.- |
| Enzyme Function | FUNCTION: Sensor of abasic sites in single-stranded DNA (ssDNA) required to preserve genome integrity by promoting error-free repair of abasic sites (PubMed:30554877). Recognizes and binds abasic sites in ssDNA at replication forks and chemically modifies the lesion by forming a covalent cross-link with DNA: forms a stable thiazolidine linkage between a ring-opened abasic site and the alpha-amino and sulfhydryl substituents of its N-terminal catalytic cysteine residue (PubMed:30554877, PubMed:31235915, PubMed:31504793). May act as a protease: mediates autocatalytic processing of its N-terminal methionine in order to expose the catalytic cysteine (By similarity). {ECO:0000250|UniProtKB:Q8R1M0, ECO:0000269|PubMed:30554877, ECO:0000269|PubMed:31235915, ECO:0000269|PubMed:31504793}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (12); Chain (1); Helix (11); Initiator methionine (1); Modified residue (1); Mutagenesis (18); Site (2); Turn (3) |
| Keywords | 3D-structure;Covalent protein-DNA linkage;DNA damage;DNA-binding;Hydrolase;Protease;Reference proteome;SOS response |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | MOD_RES 2; /note="Thiazolidine linkage to a ring-opened DNA abasic site"; /evidence="ECO:0000269|PubMed:31235915, ECO:0000269|PubMed:31504793" |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (9) |
| Cross Reference PDB | 2ICU; 6KBS; 6KBU; 6KBX; 6KBZ; 6KCQ; 6KIJ; 6NUA; 6NUH; |
| Mapped Pubmed ID | 16606699; |
| Motif | |
| Gene Encoded By | |
| Mass | 24,979 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |