| IED ID | IndEnz0002011251 |
| Enzyme Type ID | protease011251 |
| Protein Name |
Zinc metalloproteinase EC 3.4.24.- Hemolysin |
| Gene Name | hly |
| Organism | Renibacterium salmoninarum |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Micrococcales Micrococcaceae Renibacterium Renibacterium salmoninarum |
| Enzyme Sequence | MKKYYAVTGIALAVGMLCTTQLAGATQAADPSVGSLDSSNVVTEFSAQGNVEQITFKSAIKSAPMSSARSAQTSAIIPGLKNLFVSAPGSDFSLNDSSNNYIKRFTQNIAGIPVLGSSITEVLDGQGAVTSAIGAVTSATKGAFPADLAAGQAAALASATKIASAGKDASAISLVDQKAIWFDAVLIGKGATGSVAVPAYQFSFTTGFAESRVLTVAANDGAILNDRTDRKDINRVVCDANSKVIDLEASNADALLKCGKTQANKPTRIEGQAASSVADVNSVYNFLNDTASFYGANTKANDLTALIGNDEGDGLGKAMRAVVRICVTDSQNGEQCPFANAFWYNGQMTYGQGVTTDDITGHELTHGVTEKTNGLVYANESGAINESMSDVFGEFIDLSNGSSDDTAANRWAIGEGSSLGVIRSMKDPGKYGEPAIYKGSNWKPTATNPNDNNDQGGVHSNSGVGNKLAFLITDGQTFNGQTVTGIGIAKAAQLYWAAQRQLTANATYSSLGKALNSACSANVSNNVAGTTAANCTQVANAIKAVGIK |
| Enzyme Length | 548 |
| Uniprot Accession Number | P55111 |
| Absorption | |
| Active Site | ACT_SITE 363; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; ACT_SITE 459; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: Zinc metalloprotease with hemolytic properties. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Hemolytic activity is observed from 6 to 37 degrees Celsius for mammalian erythrocytes.; |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (1); Metal binding (3); Propeptide (1); Region (1); Signal peptide (1) |
| Keywords | Calcium;Cell wall;Hydrolase;Metal-binding;Metalloprotease;Protease;Secreted;Signal;Zinc;Zymogen |
| Interact With | |
| Induction | INDUCTION: Expression of the hemolysin is modulated by the availability of iron. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, cell wall. Note=Not secreted, but probably remains attached or associated with the cell wall. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..28; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 56,379 |
| Kinetics | |
| Metal Binding | METAL 362; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 366; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 386; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Rhea ID | |
| Cross Reference Brenda |