| IED ID | IndEnz0002011264 |
| Enzyme Type ID | protease011264 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase 30 homolog dUSP30 EC 3.4.19.12 Deubiquitinating enzyme 30 homolog Ubiquitin thioesterase 30 homolog Ubiquitin-specific-processing protease 30 homolog Ub-specific protease 30 homolog |
| Gene Name | Usp30 CG3016 |
| Organism | Drosophila melanogaster (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly) |
| Enzyme Sequence | MESEKILMAAGVTVAAVVGAFVFWGPSGSRLRQRRGQIAGLHNFGLTCFLNTLLQAMAACPQFIAWLQLYNNASPDRKSLITSMLNTLEVVNGTHATLRGDPYSPGAVLRALNALGWVIPQEEHDAHELFHVLLTCLEEEAIRPQPLGCLSDALPTDNDDNSSLAGTATPVGGFRSFSSMAAGLGASQRIGDQPNRPSSAMLTDFLNMEYDESTSLQRLVRSEAHTPDSPASVCERDGNDRLGSVLLDAVSPGTPFGFPLVSNPDSLATPMLGGERSSRPRLPQSQQQQDEGLNRRVSSSCRSLERLHRGPGRVSIWSNMMPSQVAHPFQGAMGAQIVCNGCGSKSAVRYDKFDSITLNLPPQRRTGLSLGHLLSEYITSEDLSDVKCDSCNETTTHTKSVTFAKLPACLCIHVARTVWLPTGQVCKRKDYVHFPESLSMAPYSFVQPHLNSQAGTPWGSTMSLYSSSLPMNNGVGGGEGFGTMFPKNLYRLLAVVVHSGEANSGHFVTYRRGSLRNAHRWYYTSDTIVREVSIDEVLSVPAYLLFYDRGQQRQLNLR |
| Enzyme Length | 558 |
| Uniprot Accession Number | Q9W462 |
| Absorption | |
| Active Site | ACT_SITE 48; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10093; ACT_SITE 506; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10093 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000250|UniProtKB:Q70CQ3}; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Deubiquitinating enzyme that acts as a key inhibitor of mitophagy by counteracting the action of parkin (park). {ECO:0000269|PubMed:24896179}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (1); Domain (1); Region (1); Transmembrane (1) |
| Keywords | Hydrolase;Membrane;Mitochondrion;Mitochondrion outer membrane;Protease;Reference proteome;Thiol protease;Transmembrane;Transmembrane helix;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Mitochondrion outer membrane {ECO:0000250|UniProtKB:Q70CQ3}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11102377; 14605208; 17699750; 20220848; 20371351; 21074052; 22937016; 23071443; 23275879; 23899565; 24302888; 25027767; 25644700; 26120032; 26550693; 26551273; 29809156; 30333215; 31076285; 31722958; 33988679; |
| Motif | |
| Gene Encoded By | |
| Mass | 60,880 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |