| IED ID | IndEnz0002011278 |
| Enzyme Type ID | protease011278 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase 47 EC 3.4.19.12 Deubiquitinating enzyme 47 Ubiquitin thioesterase 47 Ubiquitin-specific-processing protease 47 |
| Gene Name | usp47 |
| Organism | Xenopus laevis (African clawed frog) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amphibia Batrachia Anura Pipoidea Pipidae Xenopodinae Xenopus Xenopus Xenopus laevis (African clawed frog) |
| Enzyme Sequence | MRPGEESQLVPKEIENAADEPRVLCIVQDTTNCKSVNERITLNLPASTSVKQLYEDVSNKAGYVSSTFSLMWGNGASNTDMAALDPTPDRTLQEAGFEAGKKNFLHLTDKDGEQPQLTSDESGTADSSGLDDSSQEKFIGPLPREESVAGTSNYVSQNYSYSSLLSKSDTGYVGLVNQAMTCYLNSLLQTLFMTPEFRNALYKWEFEESEEDPVSSIPYQLQRLFVLLQTSKKRAIETTDVTRSFGWDSSEAWQQHDVQELCRVMFDALEQKWKQTEQADLINQLYQGKLKDYVKCLECGYESWRIDTFLDIPLVIRPYGSNTAFGSMEEALHAFIQPETLDGPNQYFCERCKRKCDARKGLKFLHFPYLLTLQLKRFDFDYTSMHRIKLNDRMTFPDELDMSPFIDMEDEKSPQTDSCTDSGAENEGSCHSDQMSNDFSTDDAVDEGICLETNSNIEKLNKSVAEKNSLYELFSVMVHSGSAAGGHYYACIKSFADGQWYSFNDQHVSRITQEDIKKTYGGATGNRGYYSSAFASSTNAYMLMYRLKNPARNAQFLEASEFPEHIKLLVQKEQEQEEQEKRQREIERNTCKLKLFCMHPVKQIMMESKLEVHKDKTMKEAVEIANKLMDLEGVIPLDCCRLVKYDEFHDYLERSYEDEEDRTMGYLLGGVKSSYMFDLLLETKRPDQVFQSYKPGEVMVKVYVVDLKTETVASPVSVRAYLSQTIIEFKQLISKSVDLPPDSMRVILERCYNDLRLLNVANKTLKAEGFFRSNKVFVESSDSLDHQLVFTDSHLWKLLDRHANTIKLYVSLPEHPRPTARSVGPKGGGDMNPQEDYCSRAKSVDAILEESTEKLKSLSLQQHQDGGNGDSSKSTEGSDFENIDSPLNEVDSSGSADNRELENRILPADPENNFQPEERSDSDVNNDRSTSSVDSDILSSSHSSDTLCNADGTPIPLANGLDSHSITSSRRSKANDGKKETWDTAEEDSGTDSEYDESGKSRGEAQYMYFKAEPHAGEGCLAEGSKCLLVNVDKRITLAAFKQQLESFVGVPSSQFKVFRVYASNQEFESVRLNETLSSFSDDNKITIRLGRALKKGEYRVKIFQLLVNEPEPCKFVLDAVFSKGMTVRQSKEELLPLLREQCGLDLSIDRFRLRKKTWKNPGTVFLDSHVYENESISSCWEVLLEALDETEKMKSMSQLSLFTKRWRPSELKLDPFKEVVLESNSVEELRDKICEISAIPLENLEFAKGRGTFPCDISVLEIHQDLDWNPKVSTLNAWPLYISDDGAVIFYRDKTEEVMELTDEQRNELVKKESSRLQKTGHRVTYSPRKEKALKIYLDGPTNKDSAQD |
| Enzyme Length | 1350 |
| Uniprot Accession Number | Q5U252 |
| Absorption | |
| Active Site | ACT_SITE 182; /note="Nucleophile"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093"; ACT_SITE 487; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Ubiquitin-specific protease that specifically deubiquitinates monoubiquitinated DNA polymerase beta (polb), stabilizing polb thereby playing a role in base-excision repair (BER). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (5); Domain (1); Region (4) |
| Keywords | Cytoplasm;DNA damage;DNA repair;Hydrolase;Protease;Thiol protease;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 153,261 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |