| IED ID | IndEnz0002011313 |
| Enzyme Type ID | protease011313 |
| Protein Name |
Tryptase beta-2 Tryptase-2 EC 3.4.21.59 Mast cell protease 6 mMCP-6 |
| Gene Name | Tpsb2 Mcpt6 |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MLKRRLLLLWALSLLASLVYSAPRPANQRVGIVGGHEASESKWPWQVSLRFKLNYWIHFCGGSLIHPQWVLTAAHCVGPHIKSPQLFRVQLREQYLYYGDQLLSLNRIVVHPHYYTAEGGADVALLELEVPVNVSTHIHPISLPPASETFPPGTSCWVTGWGDIDNDEPLPPPYPLKQVKVPIVENSLCDRKYHTGLYTGDDFPIVHDGMLCAGNTRRDSCQGDSGGPLVCKVKGTWLQAGVVSWGEGCAQPNKPGIYTRVTYYLDWIHRYVPEHS |
| Enzyme Length | 276 |
| Uniprot Accession Number | P21845 |
| Absorption | |
| Active Site | ACT_SITE 75; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 122; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 225; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa, but with more restricted specificity than trypsin.; EC=3.4.21.59; |
| DNA Binding | |
| EC Number | 3.4.21.59 |
| Enzyme Function | FUNCTION: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Plays a role in innate immunity. {ECO:0000269|PubMed:17456473}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Alternative sequence (2); Chain (1); Disulfide bond (4); Domain (1); Glycosylation (1); Modified residue (1); Propeptide (1); Signal peptide (1) |
| Keywords | Alternative splicing;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Phosphoprotein;Protease;Reference proteome;Secreted;Serine protease;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. Note=Released from the secretory granules upon mast cell activation. {ECO:0000250}. |
| Modified Residue | MOD_RES 98; /note=Phosphotyrosine; /evidence=ECO:0007744|PubMed:17947660 |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..21 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10521469; 10783129; 11533057; 1372640; 14578624; 14583634; 14706770; 15265919; 15451032; 16257142; 16807746; 18354212; 18668540; 19109198; 20832582; 20864640; 21076070; 21173247; 21267068; 21493897; 22481124; 23380220; 23573292; 23624557; 23817418; 23975861; 24257755; 24478313; 24507159; 24726923; 24814421; 25320274; 26917739; 28990934; 29429965; 31894627; 7849408; 7919662; 8098710; 8537314; 8839840; 8996238; 9064323; 9398854; 9469453; 9541598; |
| Motif | |
| Gene Encoded By | |
| Mass | 30,927 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |