| IED ID | IndEnz0002011335 |
| Enzyme Type ID | protease011335 |
| Protein Name |
Cysteine protease YopT EC 3.4.22.- |
| Gene Name | yopT |
| Organism | Yersinia enterocolitica |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Yersiniaceae Yersinia Yersinia enterocolitica |
| Enzyme Sequence | MDSIHGHYHIQLSNYSAGENLQSATLTEGVIGAHRVKVETALSHSNRQKKLSATIKHNQSSRSMLDRKLTSDGKANQRSSFTFSMIMYRMIHFVLSTRVPAVRESVANYGGNINFKFAQTKGAFLHQIIKHSDTASGVCEALCAHWIWSHAQGQSLFDQLYVGGRKGKFQIDTLYSIKQLQIDGCKADVDQDEVTLDWFKKKGISERMIERHCLLRPVDVTGTTESEGPDQLLNAILDTHGIGYGYKKIYLSGQMSAHAIAAYVNEKSGVTFFDPNFGEFHFSDKEKFRKWFTNSFWENSMYHYPLGVGQRFSVLTFDSKEV |
| Enzyme Length | 322 |
| Uniprot Accession Number | P27475 |
| Absorption | |
| Active Site | ACT_SITE 139; /evidence=ECO:0000250; ACT_SITE 258; /evidence=ECO:0000250; ACT_SITE 274; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.22.- |
| Enzyme Function | FUNCTION: Cysteine protease, which is translocated into infected cells and plays a central role in pathogenesis by cleaving the C-terminus end of the human small GTPase RhoA/ARHA, a regulator of cytoskeleton. Once cleaved, ARHA loses its lipid modification, and is released from the cell membrane, leading to the subsequent disruption of actin cytoskeleton of the host cell (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Compositional bias (1); Region (1) |
| Keywords | Hydrolase;Plasmid;Protease;Secreted;Thiol protease;Virulence |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:9723929}. Note=In infected cells, it is cytoplasmic. Translocated into the host cell by the type III secretion apparatus with the help of the SycT chaperone. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | Plasmid pYVe227; Plasmid pYV |
| Mass | 36,439 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |