| IED ID | IndEnz0002011336 |
| Enzyme Type ID | protease011336 |
| Protein Name |
Trypsin-1 EC 3.4.21.4 Trypsin I |
| Gene Name | |
| Organism | Salmo salar (Atlantic salmon) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Actinopterygii Actinopteri Neopterygii Teleostei Osteoglossocephalai Clupeocephala Euteleosteomorpha Protacanthopterygii Salmoniformes (salmons and trouts) Salmonidae (salmonids) Salmoninae (trouts salmons & chars) Salmo Salmo salar (Atlantic salmon) |
| Enzyme Sequence | MISLVFVLLIGAAFATEDDKIVGGYECKAYSQTHQVSLNSGYHFCGGSLVNENWVVSAAHCYKSRVEVRLGEHNIKVTEGSEQFISSSRVIRHPNYSSYNIDNDIMLIKLSKPATLNTYVQPVALPTSCAPAGTMCTVSGWGNTMSSTADSNKLQCLNIPILSYSDCNNSYPGMITNAMFCAGYLEGGKDSCQGDSGGPVVCNGELQGVVSWGYGCAEPGNPGVYAKVCIFNDWLTSTMASY |
| Enzyme Length | 242 |
| Uniprot Accession Number | P35031 |
| Absorption | |
| Active Site | ACT_SITE 60; /note=Charge relay system; ACT_SITE 104; /note=Charge relay system; ACT_SITE 196; /note=Charge relay system |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.; EC=3.4.21.4; |
| DNA Binding | |
| EC Number | 3.4.21.4 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (14); Chain (1); Disulfide bond (6); Domain (1); Helix (4); Metal binding (4); Natural variant (1); Propeptide (1); Signal peptide (1); Site (1); Turn (4) |
| Keywords | 3D-structure;Calcium;Digestion;Disulfide bond;Hydrolase;Metal-binding;Protease;Reference proteome;Secreted;Serine protease;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, extracellular space. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..15; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (10) |
| Cross Reference PDB | 1BIT; 1BZX; 1HJ8; 1UTJ; 1UTK; 1UTL; 1UTM; 2STA; 2STB; 2TBS; |
| Mapped Pubmed ID | 10089404; 11264577; 15044735; 15299802; 9760170; |
| Motif | |
| Gene Encoded By | |
| Mass | 25,958 |
| Kinetics | |
| Metal Binding | METAL 72; /note=Calcium; METAL 74; /note=Calcium; via carbonyl oxygen; METAL 77; /note=Calcium; via carbonyl oxygen; METAL 82; /note=Calcium |
| Rhea ID | |
| Cross Reference Brenda |