| IED ID | IndEnz0002011445 |
| Enzyme Type ID | protease011445 |
| Protein Name |
Zinc metalloproteinase-disintegrin-like daborhagin-M EC 3.4.24.- Snake venom metalloproteinase SVMP Fragment |
| Gene Name | |
| Organism | Daboia siamensis (Eastern Russel's viper) (Daboia russelii siamensis) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Lepidosauria (lepidosaurs) Squamata (squamates) Bifurcata (split-tongued squamates) Unidentata Episquamata Toxicofera Serpentes (snakes) Colubroidea Viperidae Viperinae (vipers) Daboia Daboia siamensis (Eastern Russel's viper) (Daboia russelii siamensis) |
| Enzyme Sequence | VATSEPNRYFNPYSYVELIITVDHS |
| Enzyme Length | 25 |
| Uniprot Accession Number | P0DJH5 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by EDTA, EGTA and 1,10-phenanthroline. Addition of Mg(2+) or Ca(2+) increases the casein hydrolysis rate. {ECO:0000269|PubMed:18554518}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: Snake venom zinc metalloprotease that possesses high hemorrhagic activity (minimum hemorrhagic dose, MHD=0.86 ug) when subcutaneously injected into mice. Has potent fibrinogenolytic activity on alpha-chain of fibrinogen (FGA). Hydrolyzes model substrate (beta-chain of insulin) at Ala(14)-Leu(15) and Tyr(16)-Leu(17) followed by His(10)-Leu(11) and Phe(24)-Phe(25). {ECO:0000269|PubMed:18554518}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Domain (1); Metal binding (1); Non-terminal residue (1) |
| Keywords | Calcium;Direct protein sequencing;Disulfide bond;Fibrinogenolytic toxin;Glycoprotein;Hemorrhagic toxin;Hemostasis impairing toxin;Hydrolase;Metal-binding;Metalloprotease;Protease;Secreted;Toxin;Zinc;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | PTM: N-glycosylated.; PTM: Contains 16 disulfide bonds. {ECO:0000250}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 2,915 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=7.0 uM for NFF-2 (fluorogenic substrates with cleavage at Ala-Nva); |
| Metal Binding | METAL 17; /note=Calcium; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda |