| IED ID | IndEnz0002011447 |
| Enzyme Type ID | protease011447 |
| Protein Name |
Trypsin-3 EC 3.4.21.4 |
| Gene Name | TRYP3 AGAP008294 |
| Organism | Anopheles gambiae (African malaria mosquito) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Nematocera Culicomorpha Culicoidea Culicidae (mosquitos) Anophelinae Anopheles Cellia Pyretophorus gambiae species complex Anopheles gambiae (African malaria mosquito) |
| Enzyme Sequence | MISNKIAILLAVLVVAVACAQARVALKHRSVQALPRFLPRPKYDVGHRIVGGFEIDVSETPYQVSLQYFNSHRCGGSVLNSKWILTAAHCTVNLQPSSLAVRLGSSRHASGGTVVRVARVLEHPNYDDSTIDYDFSLMELESELTFSDVVQPVSLPDQDEAVEDGTMTIVSGWGNTQSAAESNAILRAANVPTVNQKECTIAYSSSGGITDRMLCAGYKRGGKDACQGDSGGPLVVDGKLVGVVSWGFGCAMPGYPGVYARVAVVRDWVRENSGA |
| Enzyme Length | 275 |
| Uniprot Accession Number | P35037 |
| Absorption | |
| Active Site | ACT_SITE 89; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 134; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 230; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.; EC=3.4.21.4; |
| DNA Binding | |
| EC Number | 3.4.21.4 |
| Enzyme Function | FUNCTION: Constitutive trypsin that is expressed 2 days after emergence, coinciding with host seeking behavior of the female. {ECO:0000269|PubMed:7498434}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (3); Domain (1); Erroneous gene model prediction (1); Propeptide (1); Sequence conflict (6); Signal peptide (1); Site (1) |
| Keywords | Digestion;Disulfide bond;Hydrolase;Protease;Reference proteome;Secreted;Serine protease;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:7498434}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..22; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 29,374 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |