| IED ID | IndEnz0002011448 |
| Enzyme Type ID | protease011448 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase 51 EC 3.4.19.12 Deubiquitinating enzyme 51 |
| Gene Name | Usp51 |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MRGTQGAQEMKPELWPEPKPTSENLTSRGSGSYEKVLPSIPAACHTSSSSVCPRRKPRPRPQPRSRSRGGRGLKAPPPPPAKPPPPPPAPPPPPLPKQRSVAWRNSRRRSRPGPRPQTRKSYSSDHGSSRDSDGSENSLLEVGSNKGPTGCCHVESFKVAKNWQRNLRMIYQRFIWSGTPETRKRKAKSCICQICSTHKNRLHSCLSCVFFGCFTDKHIHIHAETTQHNLAVDLCHGVIYCFMCRDYVYDKDIEKIAKETKEKILGLLSSPTGDASYQQLMASEVEENQLTCESKDQETSLVKPKKKRRKKTMYYTVGFRGLINLGNTCFMNCIVQVLTHIPLLKEFFLSNKHKCMMTSPSLCLVCEMSLLFQAMYSGNQSPHIPYKLLHLIWIHAEHLAGYRQQDAQEFLIAILDVLHRHSRDDGIDQEGNSNCCNCIIDHIFTGSLQSDLTCQVCHGVSTTIDPCWDISLDLPGPYTPGRASSSTSSRDGQKPRVISLTDCLKWFTRPEDLGSSAKIKCSQCQSYQESTKQLTMKKLPIVACFHLKRFEHLGKQRRKINSFISFPLELDMTPFLASTKESIMKGQPLTECVPSENKYSLFAVINHHGTLESGHYTSFVRQEKDQWFSCDDAVVTKATMEELLNSEGYLLFYHRQDIEKE |
| Enzyme Length | 661 |
| Uniprot Accession Number | B1AY15 |
| Absorption | |
| Active Site | ACT_SITE 329; /note="Nucleophile"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01035, ECO:0000269|PubMed:27083998"; ACT_SITE 615; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01035, ECO:0000269|PubMed:27083998" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000269|PubMed:27083998}; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Deubiquitinates histone H2A at 'Lys-13' and 'Lys-15' and regulates DNA damage response. USP51 is recruited to chromatin after DNA damage and regulates the dynamic assembly/disassembly of TP53BP1 and BRCA1 foci. {ECO:0000269|PubMed:27083998}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (1); Domain (1); Metal binding (12); Mutagenesis (2); Region (1); Zinc finger (1) |
| Keywords | Chromosome;DNA damage;DNA repair;Hydrolase;Metal-binding;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway;Zinc;Zinc-finger |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Chromosome {ECO:0000250|UniProtKB:Q70EK9}. Note=Dissociates from chromatin immediately after DNA damage and reassociates with chromatin following DNA repair. {ECO:0000250|UniProtKB:Q70EK9}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 16141072; 21267068; |
| Motif | |
| Gene Encoded By | |
| Mass | 74,783 |
| Kinetics | |
| Metal Binding | METAL 151; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 153; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 192; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 195; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 205; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 208; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 213; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 218; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 222; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 228; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 241; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 244; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502 |
| Rhea ID | |
| Cross Reference Brenda |