| IED ID | IndEnz0002011473 |
| Enzyme Type ID | protease011473 |
| Protein Name |
Zinc metalloproteinase/disintegrin Cleaved into: Snake venom metalloproteinase atrolysin-E SVMP EC 3.4.24.44 Hemorrhagic metalloproteinase HT-E Hemorrhagic toxin E ; Disintegrin atrolysin-E Atrolysin-E disintegrin Atrolysin E/D ; Disintegrin atrolysin-E 4-73 ; Disintegrin atrolysin-E 3-73 |
| Gene Name | |
| Organism | Crotalus atrox (Western diamondback rattlesnake) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Lepidosauria (lepidosaurs) Squamata (squamates) Bifurcata (split-tongued squamates) Unidentata Episquamata Toxicofera Serpentes (snakes) Colubroidea Viperidae Crotalinae (pit vipers) Crotalus Crotalus atrox (Western diamondback rattlesnake) |
| Enzyme Sequence | MIQVLLVTICLAAFPYQGSSIILESGNVNDYEVIYPRKVTALPKGAVQPKYEDTMQYELKVNGEPVVLHLEKNKGLFSKDYSETHYSFDGRKITTNPSVEDHCYYHGRIENDADSTASISACNGLKGHFKLQGEMYLIEPLKLSDSEAHAVFKLKNVEKEDEAPKMCGVTQNWESYEPIKKASDLNLNPEHQRYVELFIVVDHGMYTKYNGDSDKIRQRVHQMVNIMKESYTYMYIDILLAGIEIWSNGDLINVQPASPNTLNSFGEWRETDLLKRKSHDNAQLLTSIAFDEQIIGRAYIGGICDPKRSTGVVQDHSEINLRVAVTMTHELGHNLGIHHDTDSCSCGGYSCIMSPVISDEPSKYFSDCSYIQCWEFIMNQKPQCILKKPLRTDTVSTPVSGNELLEAGIECDCGSLENPCCYATTCKMRPGSQCAEGLCCDQCRFMKKGTVCRVSMVDRNDDTCTGQSADCPRNGLYG |
| Enzyme Length | 478 |
| Uniprot Accession Number | P34182 |
| Absorption | |
| Active Site | ACT_SITE 330; /evidence="ECO:0000255|PROSITE-ProRule:PRU00276, ECO:0000255|PROSITE-ProRule:PRU10095" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Cleavage of 3-Asn-|-Gln-4, 9-Ser-|-His-10 and 14-Ala-|-Leu-15 bonds in insulin B chain and 14-Tyr-|-Gln-15 and 8-Thr-|-Ser-9 in A chain. Cleaves type IV collagen at 73-Ala-|-Gln-74 in alpha-1-(IV) and at 7-Gly-|-Leu-8 in alpha-2-(IV).; EC=3.4.24.44; |
| DNA Binding | |
| EC Number | 3.4.24.44 |
| Enzyme Function | FUNCTION: [Snake venom metalloproteinase atrolysin-E]: Snake venom zinc metalloproteinase that causes hemorrhage by provoking the degradation of the sub-endothelial matrix proteins (fibronectin, laminin, type IV collagen, nidogen, and gelatins). {ECO:0000269|PubMed:2817904}.; FUNCTION: [Disintegrin atrolysin-E]: Potent inhibitor of both collagen- (IC(50)=4 nM) and ADP-induced (IC(50)=8 nM) platelet aggregation. May act by binding to the platelet receptor GPIIb/GPIIIa (ITGA2B/ITGB3). {ECO:0000269|PubMed:9637732}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (4); Disulfide bond (9); Domain (2); Metal binding (3); Motif (1); Propeptide (2); Sequence conflict (1); Signal peptide (1) |
| Keywords | Cell adhesion impairing toxin;Collagen degradation;Direct protein sequencing;Disulfide bond;Hemorrhagic toxin;Hemostasis impairing toxin;Hydrolase;Metal-binding;Metalloprotease;Platelet aggregation inhibiting toxin;Protease;Secreted;Signal;Toxin;Zinc;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..20; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 456..458; /note=Cell attachment site; atypical (MVD) |
| Gene Encoded By | |
| Mass | 53,638 |
| Kinetics | |
| Metal Binding | METAL 329; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU00276; METAL 333; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU00276; METAL 339; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU00276 |
| Rhea ID | |
| Cross Reference Brenda | 3.4.24.1; |