| IED ID | IndEnz0002011486 |
| Enzyme Type ID | protease011486 |
| Protein Name |
Myofibril-bound serine protease MBSP EC 3.4.21.- Fragment |
| Gene Name | |
| Organism | Cyprinus carpio (Common carp) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Actinopterygii Actinopteri Neopterygii Teleostei Osteoglossocephalai Clupeocephala Otomorpha Ostariophysi Otophysi Cypriniphysae Cypriniformes (carps and others) Cyprinoidei Cyprinidae Cyprininae Cyprinus Cyprinus carpio (Common carp) |
| Enzyme Sequence | IIGGYECKPHSQPWQAFLVDNK |
| Enzyme Length | 22 |
| Uniprot Accession Number | P84477 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by the serine protease inhibitors, antipain, aprotinin, DFP, leupeptin, STI and TLCK, and by the cysteine proteinase inhibitors DTNB and to a lesser extent E-64. Not inhibited by the metalloproteinase inhibitor EDTA. {ECO:0000269|PubMed:9159882}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Serine protease that selectively cleaves Arg-|-Xaa bonds. {ECO:0000269|PubMed:9159882}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 55 degrees Celsius. {ECO:0000269|PubMed:9159882}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 8.0. Active from pH 5.0 to at least 9.0. {ECO:0000269|PubMed:9159882}; |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Non-terminal residue (1) |
| Keywords | Cytoplasm;Direct protein sequencing;Hydrolase;Protease;Serine protease |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:9159882}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 2,531 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |