| IED ID | IndEnz0002011491 |
| Enzyme Type ID | protease011491 |
| Protein Name |
Replicase polyprotein EC 2.7.7.48 EC 3.4.22.- |
| Gene Name | ORF1 |
| Organism | Acute bee paralysis virus (strain Rothamsted) (ABPV) |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Pisuviricota Pisoniviricetes Picornavirales Dicistroviridae Aparavirus Acute bee paralysis virus Acute bee paralysis virus (strain Rothamsted) (ABPV) |
| Enzyme Sequence | MNFTKQPASLKYLSSMKLITSQDQDFFNFGEVSREFILEQAYVNGYDFHMLHQDMNCIGFVLSIFDEDARDEYEYKDLNCEYHENLFDAVVDSEFDKIWPHLVLKYITYYPCSLNWRGMPTIPIVYVSKHFWYELYRTGFLNKLYHCGSWTDILLLLSGDVETNPGPVETYKDLCRRKNIRKRKSRIREEIKMQQHIDKIIGQENEEYKIINVNMQGIFSFNEEKEIIKSTAWKFNSTLDKTNSIIDNLIPQLEETLAGFRKTYSKCESKIFGTISVVDVCVDLISALLQVSFAKPAMKIASLAVEVFRLIKKYVSNININIDKIKELLSYGKVALNNNNPIIHVTMQSNSPILEVLLQPNIIVSAIFIALSVVFHKKFTYKKLGIEAMIKRLGDLGRAAKGCSDLNVVLNQAITNHMLEHFGKNVLGLKQEDELKVLVEGYRNWCDEVRDLVGHKINSDGELDSKSIVENIMKDVYEIQRIENMYKKGLEISRNIAELKLPTKLTISFNTHMRYLTEVFKSVDTSGAFGNKPRTQPIVIWLFGESGRGKSGMTWPLAIDLNNSLLDNVDEMRNFSKNIYMRNVEQEFWDNYQGQNIVCXDDFGQMRDSSSNPNPEFMELIRTANIAPYPLHMAHLEDKRKTKFTSKVIIMTSNVFEQDVNSLTFPDAFRRRVDLCAEVKNKDEFTKMCWSKSAGKMVQRLDKGKVKKITGDIHSTVPYIVDLIDPESGEVYKTGLEYEEFLDMCLEKTSQCRDDSAKLNDFLMDYAEKRANRSREIDEVCARTMDEAFVDAYDDVIDVNMQIETVDEMELIEPNKLREMIEQCSNKIVYTYEGIAVKITSLAFKLATLNYEEQWEQIKEMKYYVKVSSGVNYLKRVLSQGMKVCEEWMKEMINYVKEHPWMTVSLILGTLIGILTVVGFWKWLCSGDKKKNPIKRHFINTGNVLILPDRELNTFWKNQESLDLRDMYINRVEEHIISLLKLQHKVVLVPKVTKYILTTVENHAKISDKIILITRNRYLNYQGKFVELICGEINQFFIDPETLDTNVEAFASADLKTFVQRKPIVIEGPEFVEAQTSGDQITLRKQTQKVIEAFASSDAITMARKTPKFVESDDVVEVSMQMWKDQVAQKLITNRVLTNLYKICLVKENGDMVPLLNGLFVRSNIMLAPGHLVGFLSDSDTIEIRNLFDVVFRVPWKDVKKVDVVNAFGESKEAVLLCFPKFVCQHTDLVKHFQDSESMSKFKRCEVTLPVLRYSDKMNRFLATLIECDKVEAYDRPYTLNDSSKGQYILRQGLEYTMPTTNGDCGAPLVINETQVIRKIAGIHVAGDARGKAYAESISQKDLIRAFSKIDVSMQIQLDLDQTLNFNQQQXIIPPNAEFGPEDLDFCDLPSLKMIPVGRLSEPLFEPGKTDIRPSLVYGKISEIKTKPAILRNVIVDGKIVNIKHKNLKKCAMDTPYVSKEMTEEAFQLVKSVWLKGMRNELKKVLTYEEAICGNDSSEFISAINRSSSPGFPWIRDRIKGTKGKQGWFGAEGEYILDEDVFEAVKTRIQNAKNGVRTPVMWVDTLKDERRPIEKVDQLKTRVFSNGPMDFSITFRMYYLGFIAHLMENRITNEVSIGTNVYSQDWNKTVRKLKTMGPKVIAGDFSTFDGSLNVCIMEKFADLANEFYDDGSENALIRHVLLMDVYNSTHICGDSVYMMTHSQPSGNPATTPLNCFINSMGLRMVFELCSKKYSALNGTKCYVMKDFSKHVSIVSYGDDNVINFSDEVSEWFNMETITEAFEKLGFTYTDELKGKNGEVPKWRTIEDVQYLKRKFRYDSKRKVWEAPLCMDTILEMPNWCRGSLDIQEGTKVNCENAIMELSMHEEYVFDKWSKVISKAYQKATGDCLDISTYNGYAQERFLNYYL |
| Enzyme Length | 1906 |
| Uniprot Accession Number | Q9DSN9 |
| Absorption | |
| Active Site | ACT_SITE 1171; /note=For picornain 3C-like protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01222; ACT_SITE 1213; /note=For picornain 3C-like protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01222; ACT_SITE 1305; /note=For picornain 3C-like protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01222 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539}; |
| DNA Binding | |
| EC Number | 2.7.7.48; 3.4.22.- |
| Enzyme Function | FUNCTION: Replicase polyprotein contains helicase, VPg, protease and RNA-directed RNA polymerase functions.; FUNCTION: RNA-directed RNA polymerase replicates genomic and antigenomic RNA and transcribes the vial genome.; FUNCTION: The protease generates mature viral proteins from the precursor polyprotein.; FUNCTION: VPg is covalently linked to the 5'-end of genomic RNA. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 544..551; /note=ATP; /evidence=ECO:0000255|PROSITE-ProRule:PRU00551 |
| Features | Active site (3); Chain (1); Domain (3); Nucleotide binding (1); Region (1) |
| Keywords | ATP-binding;Hydrolase;Nucleotide-binding;Nucleotidyltransferase;Protease;RNA-directed RNA polymerase;Reference proteome;Thiol protease;Transferase;Viral RNA replication |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | PTM: Specific enzymatic cleavages in vivo by the viral protease yield a variety of precursors and mature proteins. {ECO:0000305}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 219,430 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:21248 |
| Cross Reference Brenda |