| IED ID | IndEnz0002011509 |
| Enzyme Type ID | protease011509 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase 45 EC 3.4.19.12 Ubiquitin specific peptidase 45 |
| Gene Name | usp45 |
| Organism | Danio rerio (Zebrafish) (Brachydanio rerio) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Actinopterygii Actinopteri Neopterygii Teleostei Osteoglossocephalai Clupeocephala Otomorpha Ostariophysi Otophysi Cypriniphysae Cypriniformes (carps and others) Cyprinoidei Danionidae Danioninae Danio Danio rerio (Zebrafish) (Brachydanio rerio) |
| Enzyme Sequence | MRLKDPFSLKTADMTKRSNKPKKPRDEDSSDEVGGLTCQHVSRAVDLSSVKKAVTGSLWSVCSDCLKERNVLEGETAGAHDILVCLKCGFQGCNQAEVQHSTKHQQAHHSDSHCITISLTTWKAWCFECKEELSTHCNKKALAQTLDFLQKQSAKATSGTSSKLIKLREEPAEYVDTQRGKSPVNSTLIPVKGINNLGNTCFFNAVMQNLSQTHMLNDLIQDVKEKGHKMKISPSTETNLGSLTVTLPSPEPLTSAMFLFLQSMKESGKGPVSPKILFNQLCQKAPRFKGYQQQDSQELLHYLLDSMRVEETKRIKAGILKAFNNPTEKTADEETKRQVKAYGKEGVKLNFVDRIFVGELTSTIMCEECEHISTVKEAFIDISLPIIEERISKPTNPARLGKSGREQDSLTSHDDSLAAHSQANRNSRRLSGQKLQSRHSSTSHDDRGPDTVSSRQEEDLCVAGRGLSSYRTDTMGSQSDCSEKESNLQDSSNDADSEASESEWSPRIPSVSSHSSTSDKTSITTTLSTTTHNPSLKSNPSSTPLPSIRPQQGGAVEQLVSAVSKLGLVHTSTDTLPISHSQEELSETRDRDRQHHQGAFQVLCHSYTPSSKECSVQSCLHQFTSIELLMGNNKLLCENCTDRRQRQMKRSDKKAEKVYTSARKQMLISALPPVVTLHLKRFHQAGMNLRKVNRHVDFPLLLDLAPFCSATCKNLGSGERVLYSLYGIVEHSGSMRGGHYAAYVKVRTPQRKPEQRRNQSGSREACSAPQGQWVYVSDTTVQTVPESRVLNSQAYLLFYEELL |
| Enzyme Length | 803 |
| Uniprot Accession Number | E9QG68 |
| Absorption | |
| Active Site | ACT_SITE 201; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU01035; ACT_SITE 739; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01035 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000250|UniProtKB:Q70EL2}; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Catalyzes the deubiquitination of SPDL1 (By similarity). Plays a role in the repair of UV-induced DNA damage via deubiquitination of ERCC1, promoting its recruitment to DNA damage sites (By similarity). May be involved in the maintenance of photoreceptor function (PubMed:30573563). May play a role in normal retinal development (PubMed:27613029). {ECO:0000250|UniProtKB:Q70EL2, ECO:0000269|PubMed:27613029, ECO:0000269|PubMed:30573563}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (4); Domain (1); Metal binding (12); Region (2); Zinc finger (1) |
| Keywords | Cytoplasm;Hydrolase;Metal-binding;Nucleus;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway;Zinc;Zinc-finger |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Photoreceptor inner segment {ECO:0000269|PubMed:30573563}. Cytoplasm {ECO:0000250|UniProtKB:Q70EL2}. Nucleus {ECO:0000250|UniProtKB:Q70EL2}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 20040115; |
| Motif | |
| Gene Encoded By | |
| Mass | 89,221 |
| Kinetics | |
| Metal Binding | METAL 38; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 40; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 62; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 65; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 85; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 88; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 93; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 100; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 104; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 113; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 126; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 129; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502 |
| Rhea ID | |
| Cross Reference Brenda |