| IED ID | IndEnz0002011510 |
| Enzyme Type ID | protease011510 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase 2 EC 3.4.19.12 Deubiquitinating enzyme 2 AtUBP2 Ubiquitin thioesterase 2 Ubiquitin-specific-processing protease 2 |
| Gene Name | UBP2 At1g04860 F13M7.15 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MGKKAKKKARAPTKEIQTMEISKKVSEEPPSQAGEIAEGDVKAVKETQACVHFDKALNLEKVLDKIKSSRQIKCAECNEGVYGKRGTKAKGSKGKKDFSSSDPKSNNKAIWLCLECGCYVCGGVGLPNGPQSHVLRHSRVTRHRLVIQWENPQLRWCFPCQLLLPVEKEDNGEKKDVLSEVVKLIKGRSLNNLASSDIEDQCSGSGSITSDIKLEGAVTSDIEARDGYVVRGLVNLGNTCFFNSIMQNLLSLDRLRDHFLKENGSGVGGPLASSLRKLFTETKPEAGLKSVINPRAFFGSFCSKAPQFRGYDQHDSHELLRCLLDSLSTEESALRKKRGVSDNDEKSTTLIESVFGGETSSIVSCMECGHSSKVYEPFLDLSLPVPFKKSPPKKPQPVSRAKKAKLPPKRVPKNVSKVSKVSKVLPGMVLSELNSSGKSMAVTADSDTSCSSLAPLDNGPVLETPSVLTLDNNQASESASQSDTGFDGSWLDFIGPETSGDETNLDMQEDGIDNVITAEVNQIVPSPNIVANSSVSSGDQTLEGNTERLMQDYEEIAKAEANLDEKDVQAMQSDECPATSGISAEFSQASCIGCDPGIGESSSSVNPWDEEELPLVVADSQILYMPYKEISCNDKSVEGECEASSSFVTGDHEPQNSDFVDFGGLFDEPETTEGPVFGPPSKAEASGVGFMAFSSESDPEEIDDSDLPVSVERCLGHFTKHEILSDDNAWNCENCSKNLKLQRLREKRKSNEDESRSSNTSNGWVKENEDEGFGETEILAVKQDPNDTSCVKDHSSDGRKAARIHSADESESKGTQDEDEDSEKVITVKRDATKKVLINKAPPVLTIHLKRFSQDLRGRLSKLNGHVAFKEVIDLRQYMDSRCSGEDPPVYRLAGLVEHSGTMRGGHYVAYVRGGQRVKETDSSSTAWYNVSDAYVRQVSLEKVLHSEAYILFYERIFSQE |
| Enzyme Length | 961 |
| Uniprot Accession Number | Q8W4N3 |
| Absorption | |
| Active Site | ACT_SITE 240; /note="Nucleophile"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093"; ACT_SITE 907; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. Is involved in resistance to the arginine analog canavanine (CAN). {ECO:0000269|PubMed:11115897}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (2); Domain (1); Metal binding (12); Mutagenesis (1); Region (5); Sequence conflict (2); Zinc finger (1) |
| Keywords | Hydrolase;Metal-binding;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway;Zinc;Zinc-finger |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12663224; 15028209; 15923322; 18485060; 18515112; 28695315; |
| Motif | |
| Gene Encoded By | |
| Mass | 105,152 |
| Kinetics | |
| Metal Binding | METAL 50; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 52; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 74; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 77; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 113; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 116; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 121; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 133; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 137; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 143; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 157; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 160; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502 |
| Rhea ID | |
| Cross Reference Brenda |