| IED ID | IndEnz0002011555 |
| Enzyme Type ID | protease011555 |
| Protein Name |
Snake venom metalloproteinase patagonfibrase SVMP EC 3.4.24.- Fragment |
| Gene Name | |
| Organism | Philodryas patagoniensis (Ringless green snake) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Lepidosauria (lepidosaurs) Squamata (squamates) Bifurcata (split-tongued squamates) Unidentata Episquamata Toxicofera Serpentes (snakes) Colubroidea Dipsadidae Philodryas Philodryas patagoniensis (Ringless green snake) |
| Enzyme Sequence | LSTDIVAPPV |
| Enzyme Length | 10 |
| Uniprot Accession Number | P85103 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by DTT, EDTA and L-cysteine, but not by the serine protease inhibitor PMSF. Activated by calcium, unaffected by magnesium, inhibited by zinc. {ECO:0000269|PubMed:17306461}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: Snake venom zinc metalloprotease that hydrolyzes the alpha chain of fibrinogen (FGA) but not the beta or gamma chains. Has caseinolytic activity. Induces hemorrhage after intradermal injection into mice. Intramuscular injection into the gastrocnemius muscle of mice causes hemorrhage, edema, myonecrosis, increase in serum creatine kinase levels and multifocal lung hemorrhage. Inhibits collagen-induced platelet aggregation, but not thrombin- or ristocetin-induced platelet aggregation. {ECO:0000269|PubMed:17306461}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 37 degrees Celsius with azocasein as substrate. Incubation at 55 degrees Celsius for 90 minutes reduces activity to 20% of its maximum. {ECO:0000269|PubMed:17306461}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7.5 with azocasein as substrate. Incubation at a pH of below 6.0 or above 9.5 results in an abrupt decrease in activity. {ECO:0000269|PubMed:17306461}; |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Metal binding (1); Non-terminal residue (2) |
| Keywords | Calcium;Direct protein sequencing;Hemorrhagic toxin;Hemostasis impairing toxin;Hydrolase;Metal-binding;Metalloprotease;Platelet aggregation inhibiting toxin;Protease;Secreted;Toxin |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:17306461}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 1,011 |
| Kinetics | |
| Metal Binding | METAL 10; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda |