| IED ID | IndEnz0002011593 |
| Enzyme Type ID | protease011593 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase 7 EC 3.4.19.12 Deubiquitinating enzyme 7 Ubiquitin thioesterase 7 Ubiquitin-specific-processing protease 7 |
| Gene Name | USP7 |
| Organism | Gallus gallus (Chicken) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Archelosauria Archosauria Dinosauria Saurischia Theropoda Coelurosauria Aves Neognathae Galloanserae Galliformes Phasianidae (turkeys) Phasianinae Gallus Gallus gallus (Chicken) |
| Enzyme Sequence | MNHHQQQQHQKPGEQQLSEPEDMEMEAGDADDPPRITQNPVINGNVAMADGHNNTEEDMEDDTSWRSEATFQFTVERFNRLSESVLSPPCFVRNLPWKIMVMPRLYPDRPHQKSVGFFLQCNAESDSTSWSCHAQAVLKIINYKDDEKSFSRRISHLFFHKENDWGFSNFMAWSEVTDPEKGFIEEDKVTFEVYVQADAPHGVAWDSKKHTGYVGLKNQGATCYMNSLLQTLFFTNQLRKAVYMMPTEGDDSSKSVPLALQRVFYELQHSDKPVGTKKLTKSFGWETLDSFMQHDVQELCRVLLDNVENKMKGTCVEGTIPKLFRGKMVSYIQCKHVDYRSERIEDYYDIQLSIKGKKNIFESFIDYVAVEQLDGDNKYDAGEHGLQEAEKGVKFLTLPPVLHLQLMRFMYDPQTDQNIKINDRFEFPEQLPLDEFLQKTDPKDPANYILHAVLVHSGDNHGGHYVVYLNPKGDGKWCKFDDDVVSRCTKEEAIEHNYGGHDDDLSVRHCTNAYMLVYIRESKLSEVLQPVTDHDIPQQLVERLQEEKRIEAQKRKERQEAHLYMQVQIVAEDQFCGHQGNDMYDEEKVKYTVFKVLKNSTLTEFVQNLSQTMGFPQDQIRLWPMQARSNGTKRPAMLDNEADGNKTMIELSDNENPWTIFLETVDPEMAATGATLPKFDKDHDVMLFLKMYDPKTRSLNYCGHIYTPISCKIRDLLPVMCERAGFPQETNLILYEEVKPNLTERIQDYDVSLDKALDELMDGDIIVFQKDDPENDSSELPTAKEYFRDLYSRVDVIFCDKTIPNDPGFVVTLSNRMNYFQVAKTVAQRLNTDPMLLQFFKSQGYRDGPGNPLRHNYEGTLRDLLQFFKPRQPKKLYYQQLKMKITDFENRRSFKCTWLNSQFREEEITVYPDKHGCVRDLLEECKKVVELSEKGSGKLRLLEIVSYKIIGVHQEDELLECLSPATSRTFRIEEIPLAQVDIDKENEMLITVAHFHKEVFGTFGIPFLLRIHQGGHFREVMKRIQTMLDIQEKEFEKFKFAIVMMGRHTYLNEDEYEVNLKDFEPQPGNMSHPRPWLGLDHFNKAPKDRYTYLEKAYKIHN |
| Enzyme Length | 1101 |
| Uniprot Accession Number | Q6U7I1 |
| Absorption | |
| Active Site | ACT_SITE 223; /note="Nucleophile"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093"; ACT_SITE 464; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000250|UniProtKB:Q93009}; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Hydrolase that deubiquitinates target proteins, such as p53/TP53. Deubiquitinates p53/TP53, and thereby modulates p53/TP53 stability, p53/TP53-dependent transcription regulation, cell growth repression and apoptosis. May be involved in cell proliferation during early embryonic development (By similarity). {ECO:0000250|UniProtKB:Q93009}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Alternative sequence (4); Chain (1); Domain (2); Region (3) |
| Keywords | Alternative splicing;Chromosome;Cytoplasm;Developmental protein;Hydrolase;Nucleus;Phosphoprotein;Protease;Reference proteome;Thiol protease;Ubl conjugation;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q93009}. Cytoplasm {ECO:0000250|UniProtKB:Q93009}. Nucleus, PML body {ECO:0000250|UniProtKB:Q93009}. Chromosome {ECO:0000250|UniProtKB:Q93009}. |
| Modified Residue | |
| Post Translational Modification | PTM: Phosphorylated. {ECO:0000250}.; PTM: Polyneddylated. {ECO:0000250}.; PTM: Polyubiquitinated. {ECO:0000250}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 128,297 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |