| IED ID | IndEnz0002011624 |
| Enzyme Type ID | protease011624 |
| Protein Name |
Cysteine protease XCP2 EC 3.4.22.- Xylem cysteine peptidase 2 AtXCP2 |
| Gene Name | XCP2 At1g20850 F2D10.37 F9H16.17 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MALSSPSRILCFALALSAASLSLSFASSHDYSIVGYSPEDLESHDKLIELFENWISNFEKAYETVEEKFLRFEVFKDNLKHIDETNKKGKSYWLGLNEFADLSHEEFKKMYLGLKTDIVRRDEERSYAEFAYRDVEAVPKSVDWRKKGAVAEVKNQGSCGSCWAFSTVAAVEGINKIVTGNLTTLSEQELIDCDTTYNNGCNGGLMDYAFEYIVKNGGLRKEEDYPYSMEEGTCEMQKDESETVTINGHQDVPTNDEKSLLKALAHQPLSVAIDASGREFQFYSGGVFDGRCGVDLDHGVAAVGYGSSKGSDYIIVKNSWGPKWGEKGYIRLKRNTGKPEGLCGINKMASFPTKTK |
| Enzyme Length | 356 |
| Uniprot Accession Number | Q9LM66 |
| Absorption | |
| Active Site | ACT_SITE 162; /evidence=ECO:0000255|PROSITE-ProRule:PRU10088; ACT_SITE 298; /evidence=ECO:0000255|PROSITE-ProRule:PRU10089; ACT_SITE 318; /evidence=ECO:0000255|PROSITE-ProRule:PRU10090 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.22.- |
| Enzyme Function | FUNCTION: Cysteine protease involved in xylem tracheary element (TE) autolysis during xylogenesis in roots. Participates in micro autolysis within the intact central vacuole before mega autolysis is initiated by tonoplast implosion (PubMed:18573193). Involved in susceptibility to the bacterial plant pathogen Ralstonia solanacearum (PubMed:24947605). {ECO:0000269|PubMed:18573193, ECO:0000269|PubMed:24947605}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (3); Glycosylation (1); Propeptide (1); Signal peptide (1) |
| Keywords | Cell membrane;Disulfide bond;Glycoprotein;Hydrolase;Membrane;Plant defense;Protease;Reference proteome;Signal;Thiol protease;Vacuole;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Vacuole {ECO:0000250|UniProtKB:O65493}. Cell membrane {ECO:0000250|UniProtKB:O65493}. Note=Predominantly vacuolar. May be associated to plasma membrane. {ECO:0000250|UniProtKB:O65493}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..26; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 14585825; 14697270; 15272873; 15593128; 15763661; 15923329; 16061508; 16081527; 16214898; 16299169; 16367961; 16927204; 17565617; 17890373; 20736450; 21798944; 22371507; |
| Motif | |
| Gene Encoded By | |
| Mass | 39,708 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |