| IED ID | IndEnz0002011675 |
| Enzyme Type ID | protease011675 |
| Protein Name |
Intestinal-type alkaline phosphatase 1 IAP-1 Intestinal alkaline phosphatase 1 EC 3.1.3.1 Intestinal alkaline phosphatase I IAP-I |
| Gene Name | Alpi |
| Organism | Rattus norvegicus (Rat) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Rattus Rattus norvegicus (Rat) |
| Enzyme Sequence | MQGDWVLLLLLGLRIHLSFGVIPVEEENPVFWNQKAKEALDVAKKLQPIQTSAKNLILFLGDGMGVPTVTATRILKGQLGGHLGPETPLAMDHFPFTALSKTYNVDRQVPDSAGTATAYLCGVKANYKTIGVSAAARFNQCNSTFGNEVFSVMHRAKKAGKSVGVVTTTRVQHASPAGTYAHTVNRDWYSDADMPSSALQEGCKDIATQLISNMDIDVILGGGRKFMFPKGTPDPEYPGDSDQSGVRLDSRNLVEEWLAKYQGTRYVWNREQLMQASQDPAVTRLMGLFEPTEMKYDVNRNASADPSLAEMTEVAVRLLSRNPQGFYLFVEGGRIDQGHHAGTAYLALTEAVMFDSAIEKASQLTNEKDTLTLITADHSHVFAFGGYTLRGTSIFGLAPLNAQDGKSYTSILYGNGPGYVLNSGNRPNVTDAESGDVNYKQQAAVPLSSETHGGEDVAIFARGPQAHLVHGVQEQNYIAHVMAFAGCLEPYTDCGLAPPADENRPTTPVQNSAITMNNVLLSLQLLVSMLLLVGTALVVS |
| Enzyme Length | 540 |
| Uniprot Accession Number | P15693 |
| Absorption | |
| Active Site | ACT_SITE 112; /note="Phosphoserine intermediate"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10042, ECO:0000269|PubMed:24076154" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a phosphate monoester + H2O = an alcohol + phosphate; Xref=Rhea:RHEA:15017, ChEBI:CHEBI:15377, ChEBI:CHEBI:30879, ChEBI:CHEBI:43474, ChEBI:CHEBI:67140; EC=3.1.3.1; Evidence={ECO:0000255|PROSITE-ProRule:PRU10042, ECO:0000269|PubMed:2155025, ECO:0000269|PubMed:24076154}; |
| DNA Binding | |
| EC Number | 3.1.3.1 |
| Enzyme Function | FUNCTION: Alkaline phosphatase that can hydrolyze various phosphate compounds. {ECO:0000269|PubMed:2155025, ECO:0000269|PubMed:24076154}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (17); Chain (1); Disulfide bond (2); Glycosylation (3); Helix (22); Lipidation (1); Metal binding (14); Propeptide (1); Signal peptide (1); Turn (3) |
| Keywords | 3D-structure;Calcium;Cell membrane;Direct protein sequencing;Disulfide bond;GPI-anchor;Glycoprotein;Hydrolase;Lipoprotein;Magnesium;Membrane;Metal-binding;Reference proteome;Signal;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:2155025}; Lipid-anchor, GPI-anchor {ECO:0000269|PubMed:2155025}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..20; /evidence=ECO:0000269|PubMed:2155025 |
| Structure 3D | X-ray crystallography (2) |
| Cross Reference PDB | 4KJD; 4KJG; |
| Mapped Pubmed ID | 15885097; 19407215; 22018098; 22405696; 27939968; 28739053; 28985873; 29723880; |
| Motif | |
| Gene Encoded By | |
| Mass | 58,402 |
| Kinetics | |
| Metal Binding | METAL 62; /note=Magnesium; /evidence=ECO:0000269|PubMed:24076154; METAL 62; /note=Zinc 1; /evidence=ECO:0000269|PubMed:24076154; METAL 112; /note=Zinc 1; /evidence=ECO:0000269|PubMed:24076154; METAL 175; /note=Magnesium; /evidence=ECO:0000269|PubMed:24076154; METAL 236; /note=Calcium; /evidence=ECO:0000250|UniProtKB:P05186; METAL 289; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:P05186; METAL 290; /note=Calcium; /evidence=ECO:0000250|UniProtKB:P05186; METAL 305; /note=Calcium; /evidence=ECO:0000250|UniProtKB:P05186; METAL 331; /note=Magnesium; /evidence=ECO:0000269|PubMed:24076154; METAL 336; /note=Zinc 2; /evidence=ECO:0000269|PubMed:24076154; METAL 340; /note=Zinc 2; via tele nitrogen; /evidence=ECO:0000269|PubMed:24076154; METAL 377; /note=Zinc 1; /evidence=ECO:0000269|PubMed:24076154; METAL 378; /note=Zinc 1; via tele nitrogen; /evidence=ECO:0000269|PubMed:24076154; METAL 452; /note=Zinc 2; via tele nitrogen; /evidence=ECO:0000269|PubMed:24076154 |
| Rhea ID | RHEA:15017 |
| Cross Reference Brenda |