| IED ID | IndEnz0002011701 |
| Enzyme Type ID | protease011701 |
| Protein Name |
Tricorn protease EC 3.4.21.- |
| Gene Name | tri Ta1490 |
| Organism | Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165) |
| Taxonomic Lineage | cellular organisms Archaea Candidatus Thermoplasmatota Thermoplasmata Thermoplasmatales Thermoplasmataceae Thermoplasma Thermoplasma acidophilum Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165) |
| Enzyme Sequence | MPSLMSFGSCQWIDQGRFSRSLYRNFKTFKLHEMHGLCMPNLLLNPDIHGDRIIFVCCDDLWEHDLKSGSTRKIVSNLGVINNARFFPDGRKIAIRVMRGSSLNTADLYFYNGENGEIKRITYFSGKSTGRRMFTDVAGFDPDGNLIISTDAMQPFSSMTCLYRVENDGINFVPLNLGPATHILFADGRRVIGRNTFELPHWKGYRGGTRGKIWIEVNSGAFKKIVDMSTHVSSPVIVGHRIYFITDIDGFGQIYSTDLDGKDLRKHTSFTDYYPRHLNTDGRRILFSKGGSIYIFNPDTEKIEKIEIGDLESPEDRIISIPSKFAEDFSPLDGDLIAFVSRGQAFIQDVSGTYVLKVPEPLRIRYVRRGGDTKVAFIHGTREGDFLGIYDYRTGKAEKFEENLGNVFAMGVDRNGKFAVVANDRFEIMTVDLETGKPTVIERSREAMITDFTISDNSRFIAYGFPLKHGETDGYVMQAIHVYDMEGRKIFAATTENSHDYAPAFDADSKNLYYLSYRSLDPSPDRVVLNFSFEVVSKPFVIPLIPGSPNPTKLVPRSMTSEAGEYDLNDMYKRSSPINVDPGDYRMIIPLESSILIYSVPVHGEFAAYYQGAPEKGVLLKYDVKTRKVTEVKNNLTDLRLSADRKTVMVRKDDGKIYTFPLEKPEDERTVETDKRPLVSSIHEEFLQMYDEAWKLARDNYWNEAVAKEISERIYEKYRNLVPLCKTRYDLSNVIVEMQGEYRTSHSYEMGGTFTDKDPFRSGRIACDFKLDGDHYVVAKAYAGDYSNEGEKSPIFEYGIDPTGYLIEDIDGETVGAGSNIYRVLSEKAGTSARIRLSGKGGDKRDLMIDILDDDRFIRYRSWVEANRRYVHERSKGTIGYIHIPDMGMMGLNEFYRLFINESSYQGLIVDVRFNGGGFVSQLIIEKLMNKRIGYDNPRRGTLSPYPTNSVRGKIIAITNEYAGSDGDIFSFSFKKLGLGKLIGTRTWGGVVGITPKRRLIDGTVLTQPEFAFWFRDAGFGVENYGVDPDVEIEYAPHDYLSGKDPQIDYAIDALIEELRNWNEELPQRPS |
| Enzyme Length | 1071 |
| Uniprot Accession Number | P96086 |
| Absorption | |
| Active Site | ACT_SITE 746; /note="Charge relay system"; /evidence="ECO:0000269|PubMed:11719810, ECO:0000269|PubMed:12470958"; ACT_SITE 965; /note="Nucleophile"; /evidence="ECO:0000269|PubMed:11719810, ECO:0000269|PubMed:12470958"; ACT_SITE 1023; /note="Charge relay system"; /evidence="ECO:0000269|PubMed:11719810" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Tricorn degrades oligopeptides (probably derived from the proteasome) and channels the products to F1, F2 and F3 proteases, which then catalyze the terminal degradation step, yielding free amino acids. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 65 degrees Celsius.; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 8.5-8.8.; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (72); Chain (1); Helix (18); Mutagenesis (6); Region (8); Site (2); Turn (12) |
| Keywords | 3D-structure;Cytoplasm;Direct protein sequencing;Hydrolase;Protease;Reference proteome;Serine protease |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (4) |
| Cross Reference PDB | 1K32; 1N6D; 1N6E; 1N6F; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 121,623 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.21.B34; |