| IED ID | IndEnz0002011705 |
| Enzyme Type ID | protease011705 |
| Protein Name |
Replication polyprotein Includes: Membrane protein; Helicase EC 3.6.4.- ; Protease; RNA-directed RNA polymerase EC 2.7.7.48 |
| Gene Name | ORF2 |
| Organism | Nora virus |
| Taxonomic Lineage | Viruses Riboviria unclassified Riboviria unclassified ssRNA viruses unclassified ssRNA positive-strand viruses Nora virus |
| Enzyme Sequence | MLIEAFINSLLQNLGIMMSFRDLVASPWILLVIAIPLCAFASSASMVREMLFRHKITENILKGTGVEELFNPFGIIIKYFLYFAILYAFIKYIRNNINVITEKVNFIRRVVSNPTGTTGRRGVLGRCVEQIVEYPTFFITMVYELQQIKNKKDLISKITMISSILKLPLGIWESTVGRMLDRPAIEGTEEMLEDVLPIVAMGLTITKTQIGDVPVESFLVNLDRNQKACENIIKRMQPLMIKMGMMKDSSYDTILQVAKEVNELSEAETWMKTTLKLNPNEFLQTQGAVRVGEIREKVATLRNKLNTLQTKELRSDKVVTECQKHLASLEVLLIEVKVLENSNQTRVKPVGVTIQGEKQIGKTNLVAILSRKICEYVQEHGDISFRNATKWTTWSRQCRDEFDTGYTGQEITYVDDAFQQKDNKDHLMWFTFISNTAVGTNQADLKQKGLPYRSKLVFTTCNKSPDKSVTIEDIEALHARFPHTICLRRNKNKMPKRGAIDESYDWVDFYYGPMSKAVSAIGSNTTSTLKTMSLSEIVKIIGDDLIIQNNFYNSTIKDVGITGQEQMDGAQLERRQRMRELRDHLLRIRSGDENMPFLDETFELNSRPIQTDEKFIPLKDNLDEEVMYGGISDQLLTRFDNIIERSLEGYDVESRELGVEPLTTLNHVRSNMLSYRAWNLINSMCINKTETFSAWLGRYITECVEGVAENLVTTKVKIRINPFTGLQLIAAKRMLQENKLIDMDEIPSTSANSYETVYDQIKNFVNDELSLMETDVVDLALAKISLSQIRSNIKRSTWLDVNDWILALKHKISGKSFAKHMDLYPSSLDSFLLTLKDWEVEDRIKFNSIYKQKVLFVQSRFSLYCWSPFISRGTRFVKVTSRFRELVDKLETGILFHEIKSVTNGIRWLGGAGNNGHVGERVRVIAHTAQFPKKSYPQNGFPINEELHREWIQLVINSDYKYHSLIGEEKVNILWNLIRLQPQREVENFKVYLEDLQASPPKTGTICAKVVNDIKAEVTSSYRQFNNYYTRLTKDGMHTLLSMLSRIGVPISDYWNDLLVDKAPAITAVTVGAITSLAIITIVKTFQYAIAGEEQSKGEKRAKQKNIATTKLQKLKFTLGKEQAEGDSIEHVKEFDGDVKFETIEKLFDHIDEHPNLNIVGLNLVAPENPIAIYAAREESYDFSFSEPRPPQWKKVVTFKEDSKRIISLQLRGEDTEDNILDEIEHAIKVSHGMPYAEWIFNGWFKKESNDNILYCVELDLVTAKTQSGPVGWTRAQTKNLKDLEIQLNKGKPIDVKSVVLGAPQASTQATDTMDVLVNKHLVKVHCLSYENLNNLALNGTQVFALASDNILIVPAHAARQNKWIRFSRATQTGHYGVAKVDERRIDFTRDIAIAIILTRAEAEQKLCELGYSIQLTNISKEKFHFPLITKYLLTADQSEVEWMNCTTLHYFAKNKTVGLGRTTSFQVSEFLCGNEYISKKLVACAQGLQSSVELSRLGDCGSPIVLASGKKAGKLIGFHGYHSPNLQTWYGAMLTVEDLGIINGVEEHFDDPWAKLITQGLPVDLPIGPEVEYVGNLIRPSLPVTNDSLDHWHKSPFADQFEEQLAPGRLNPYDSYIEGDLPTNLEGRKSLILGPNSEMAKTLPELDQGILDWIVDQLVVEQVATFKAENLLTKVSDDIDEMLDYALNGNVDNTYVRGMEVNKASGLPWSLSGSPKKSDFIDVDEATGVRSFKVNANGDALKNRVILKLQQAKMGNRILSFSSSKLKDQPIKIAQAKSGRTRVFHCIPVDLILFSGALYGPYKEAYTKAGLKCYHAVGIDPKSVGWQQLATYMTKHPNYFDADYKNYDKYLHRQVFKAVRKIQRSVIQQVCPDKWDKARAVEELDAIDTYVVDYQTVYKTNRGNKSGSYTTTIDNCLANDIYGLYAWVKTTGLRSLWDYRQNVSSVAFGDDIIKSVSDEYKDKYNYCTYRDVLNATGHIMTPGSKDGEEKPFTSFENLQFLKRGFKLENGMVLAPLLQRSIEGPFVWTDIREDQITVWVNLVQEQLIEAALWGEEYYNELCQKLKCGTNRTLNGALAVLLNTSWEVTFQKFCNRYYGIKRGDL |
| Enzyme Length | 2104 |
| Uniprot Accession Number | Q27YG9 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539}; |
| DNA Binding | |
| EC Number | 3.6.4.-; 2.7.7.48 |
| Enzyme Function | FUNCTION: The peptidase activity is involved in polyprotein maturation, possibly along with hosts proteases. Transmembrane protein may be surface viral glycoprotein. RNA-directed RNA polymerase replicates the viral genome (Probable). {ECO:0000305}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Coiled coil (1); Domain (2); Region (1); Transmembrane (2) |
| Keywords | ATP-binding;Coiled coil;Helicase;Hydrolase;Membrane;Nucleotide-binding;Nucleotidyltransferase;RNA-directed RNA polymerase;Transferase;Transmembrane;Transmembrane helix;Viral RNA replication |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | PTM: Specific enzymatic cleavages in vivo yield mature proteins. {ECO:0000305}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 239,183 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:21248 |
| Cross Reference Brenda |