| IED ID | IndEnz0002011710 |
| Enzyme Type ID | protease011710 |
| Protein Name |
Lysosomal protective protein EC 3.4.16.5 Carboxypeptidase C Carboxypeptidase L Cathepsin A Protective protein cathepsin A PPCA Protective protein for beta-galactosidase Cleaved into: Lysosomal protective protein 32 kDa chain; Lysosomal protective protein 20 kDa chain |
| Gene Name | CTSA PPGB |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MIRAAPPPLFLLLLLLLLLVSWASRGEAAPDQDEIQRLPGLAKQPSFRQYSGYLKGSGSKHLHYWFVESQKDPENSPVVLWLNGGPGCSSLDGLLTEHGPFLVQPDGVTLEYNPYSWNLIANVLYLESPAGVGFSYSDDKFYATNDTEVAQSNFEALQDFFRLFPEYKNNKLFLTGESYAGIYIPTLAVLVMQDPSMNLQGLAVGNGLSSYEQNDNSLVYFAYYHGLLGNRLWSSLQTHCCSQNKCNFYDNKDLECVTNLQEVARIVGNSGLNIYNLYAPCAGGVPSHFRYEKDTVVVQDLGNIFTRLPLKRMWHQALLRSGDKVRMDPPCTNTTAASTYLNNPYVRKALNIPEQLPQWDMCNFLVNLQYRRLYRSMNSQYLKLLSSQKYQILLYNGDVDMACNFMGDEWFVDSLNQKMEVQRRPWLVKYGDSGEQIAGFVKEFSHIAFLTIKGAGHMVPTDKPLAAFTMFSRFLNKQPY |
| Enzyme Length | 480 |
| Uniprot Accession Number | P10619 |
| Absorption | |
| Active Site | ACT_SITE 178; ACT_SITE 400; /evidence=ECO:0000250; ACT_SITE 457 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Release of a C-terminal amino acid with broad specificity.; EC=3.4.16.5; Evidence={ECO:0000255|PROSITE-ProRule:PRU10074, ECO:0000255|PROSITE-ProRule:PRU10075}; |
| DNA Binding | |
| EC Number | 3.4.16.5 |
| Enzyme Function | FUNCTION: Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins. {ECO:0000269|PubMed:1907282}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Alternative sequence (1); Beta strand (21); Chain (3); Disulfide bond (4); Glycosylation (2); Helix (19); Mutagenesis (2); Natural variant (12); Sequence conflict (2); Signal peptide (1); Turn (2) |
| Keywords | 3D-structure;Alternative splicing;Carboxypeptidase;Direct protein sequencing;Disease variant;Disulfide bond;Glycoprotein;Hydrolase;Lysosome;Protease;Reference proteome;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Lysosome. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..28; /evidence="ECO:0000269|PubMed:12665801, ECO:0000269|PubMed:1694176, ECO:0007744|PubMed:25944712" |
| Structure 3D | X-ray crystallography (12) |
| Cross Reference PDB | 1IVY; 3BP4; 3BP7; 3BXN; 4AZ0; 4AZ3; 4CI9; 4CIA; 4CIB; 4MWS; 4MWT; 6WIA; |
| Mapped Pubmed ID | 10072072; 10631941; 10748235; 10809954; 11684289; 11702224; 11813165; 11853874; 12078484; 12505983; 12649068; 12742663; 12748383; 14695530; 14962670; 15213228; 15785934; 16181339; 16314420; 16538002; 17353931; 19617632; 19666471; 19797320; 19954752; 20562859; 20711500; 22532132; 22861813; 23416715; 23495688; 23915561; 24530914; 24599961; 24769197; 25075748; 26259553; 26496610; 27177495; 30010039; 30278264; 30581499; 31192586; 32305437; 32323791; 8687433; 8692836; 8985184; 9054950; 9539769; 9545226; |
| Motif | |
| Gene Encoded By | |
| Mass | 54,466 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.16.5; |