| IED ID | IndEnz0002011731 |
| Enzyme Type ID | protease011731 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase 29 EC 3.4.19.12 Deubiquitinating enzyme 29 Ubiquitin thioesterase 29 Ubiquitin-specific-processing protease 29 |
| Gene Name | Usp29 |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MAHLKINGLVQIRSTNRSKHTRASQWKEAVIEIVERKQKVNLVVSFKLEERRRVFQLGDNVTGVVVSGELGLYHLDLTLRDDTSLLIDKLSSADVEHLKSFLDSSTPCESQQPMEPMSSQDDLESSDPFCGEHQEAACGSLNTTPESGTPLSRKMPLSMSNTTGGQKRGEKQGRKRKTEPSSSSAEVNKDIPKENTPDQKKKSRRYYSRNRGGKAEKAVTLREQEKRSNWKLEPAFNSKSYGRANLDGTILPIATCSDDRDVSIFGLEIITHNGVQSLPDPYLNQLKREGFPNLGNTCYMNSILQSVFGIPTFAKDLLTQGIPWEKVSYDDLIMPLSQLLVLKDIRDVEIKGELLTSVKKSISTVADTFSGNEQNDAHEFLSLCLDQLKLNMEKVNAMWDTERRNTCAGSAGTKRFVCPVGANFEFELHSSIICEGCGEATIKTEVSNYLSIDLHHGTKTHPLSIQKSFDLFFTPEKIEHNCEKCKNKNSVLKYTLRRLPRVLIVHLKRYQVTTDLLPVKSEQPVEISKYLNISSHCHENRKLPFPLANTSPDVSQGMMPGIFNQSMLSKKVISESCDPMVLQVGSSVDAEIQSFQIMYEDEDASEEQQQRGLESGSMLEPELVKTENRILRQKTSLATDSMMGDGYSFLPMLCEPLSIQDPGLAEMGLQEVPENPEFKNYEKINIYGKSDGRTNTELSKLYQNHGSRIKGLFLPASLASVSSQEDPEKDLSRSPELQEDDPHSFAFGSDDSKDGEMGDDLQNYRLVSVVSHFGSSPNSGHYVSDVYDFQKQAWLLYSDVQVFESSDPSIQENRLNSGYIFFYMHNEIFEELLKKASECKVLSTSKEEKRDIDYFSTLLNGLTYILEEF |
| Enzyme Length | 869 |
| Uniprot Accession Number | Q9ES63 |
| Absorption | |
| Active Site | ACT_SITE 298; /note="Nucleophile"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093, ECO:0000305|PubMed:32457395"; ACT_SITE 781; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000269|PubMed:32457395}; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Deubiquitinase involved in innate antiviral immunity by mediating 'Lys-48'-linked deubiquitination of CGAS, thereby promoting its stabilization. {ECO:0000269|PubMed:32457395}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (5); Domain (1); Mutagenesis (1); Region (2); Sequence conflict (1) |
| Keywords | Cytoplasm;Hydrolase;Immunity;Innate immunity;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm, perinuclear region {ECO:0000269|PubMed:32457395}. Note=Localizes to perinuclear region in response to herpes simplex virus-1 (HSV-1) infection. {ECO:0000269|PubMed:32457395}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10903847; 11543637; 11668390; 12466851; 14610273; 14724735; 15112106; 16141072; 16300676; 16455231; 16602821; 18799693; 19068137; 19397955; 21233130; 21267068; 22394678; 24125951; 24291790; 27187722; 27327533; 27901122; 28719641; 29734399; 30335829; 32968046; 34562326; |
| Motif | |
| Gene Encoded By | |
| Mass | 97,823 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |