| IED ID | IndEnz0002011747 |
| Enzyme Type ID | protease011747 |
| Protein Name |
Gag-Pro polyprotein Pr76Gag-Pro Cleaved into: Matrix protein p19 MA ; Capsid protein p24 CA ; Nucleocapsid protein p15-pro NC' NC-pro ; Protease PR EC 3.4.23.- ; p1; Transframe peptide TFP p8 pX |
| Gene Name | gag-pro |
| Organism | Human T-cell leukemia virus 3 (strain 2026ND) (HTLV-3) |
| Taxonomic Lineage | Viruses Riboviria Pararnavirae Artverviricota Revtraviricetes Ortervirales Retroviridae Orthoretrovirinae Deltaretrovirus Primate T-lymphotropic virus 3 Human T-cell leukemia virus 3 (HTLV-3) (Human T-lymphotropic virus 3) Human T-cell leukemia virus 3 (strain 2026ND) (HTLV-3) |
| Enzyme Sequence | MGKTYSSPINPIPKAPKGLAIHHWLNFLQAAYRLQPGPSEFDFHQLRKFLKLAIKTPVWLNPINYSVLAGLIPKNYPGRVHEIVAILIQETPAREAPPSAPLAEDPQKPPPYPEQAQEASQCLPILHPHGAPAAHRPWQMKDLQAIKQEVSSSAPGSPQFMQTIRLAVQQFDPTAKDLHDLLQYLCSSLVASLHHQQLETLIAQAETQGITGYNPLAGPLRIQANNPNQQGLRKEYQNLWLSAFSALPGNTKDPTWAAILQGPEEPFGSFVERLNVALDNGLPEGTPKDPILRSLAYSNANKECQKLLQARGQTNSPLGEMLRACQTWTPRDKNKILMVQPKKTPPPNQPCFRCGQVGHWSRDCKQPRPPPGPCPVCQDPTHWKRDCPQLKTDTRDSEDLLLDLPCEAPNVRERKNLLRGGGLASPRTILPLIPLSQQKQPTLHIQVSFSNTPPVSVQALLDTGADITVLPACLCPPDSNLQDTTVLGAGGPSTNKFKILPCPVHIHLPFRRQPVTLTACLIDINNQWTILGRDALQQCQSSLYLADQPSKVLPVLAPKLIGLEHLPPPPEVSQFPLNQSASRL |
| Enzyme Length | 584 |
| Uniprot Accession Number | Q0R5R3 |
| Absorption | |
| Active Site | ACT_SITE 462; /note=For protease activity; shared with dimeric partner; /evidence=ECO:0000255|PROSITE-ProRule:PRU10094 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.23.- |
| Enzyme Function | FUNCTION: Matrix protein p19 targets Gag, Gag-Pro and Gag-Pro-Pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex (By similarity). {ECO:0000250}.; FUNCTION: Capsid protein p24 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex. {ECO:0000250}.; FUNCTION: Nucleocapsid protein p15 is involved in the packaging and encapsidation of two copies of the genome. {ECO:0000250}.; FUNCTION: The aspartyl protease mediates proteolytic cleavages of Gag, Gag-Pro and Gag-Pro-Pol polyproteins during or shortly after the release of the virion from the plasma membrane. Cleavages take place as an ordered, step-wise cascade to yield mature proteins. This process is called maturation. Displays maximal activity during the budding process just prior to particle release from the cell. Hydrolyzes host EIF4GI in order to shut off the capped cellular mRNA translation. The resulting inhibition of cellular protein synthesis serves to ensure maximal viral gene expression and to evade host immune response (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (6); Domain (1); Initiator methionine (1); Lipidation (1); Motif (2); Peptide (1); Region (1); Site (5); Zinc finger (2) |
| Keywords | Aspartyl protease;Capsid protein;Eukaryotic host gene expression shutoff by virus;Eukaryotic host translation shutoff by virus;Host gene expression shutoff by virus;Host-virus interaction;Hydrolase;Lipoprotein;Metal-binding;Myristate;Protease;Reference proteome;Repeat;Ribosomal frameshifting;Viral nucleoprotein;Virion;Zinc;Zinc-finger |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Matrix protein p19]: Virion {ECO:0000305}.; SUBCELLULAR LOCATION: [Capsid protein p24]: Virion {ECO:0000305}.; SUBCELLULAR LOCATION: [Nucleocapsid protein p15-pro]: Virion {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | PTM: Specific enzymatic cleavages by the viral protease yield mature proteins. The polyprotein is cleaved during and after budding, this process is termed maturation. The protease is autoproteolytically processed at its N- and C-termini (By similarity). {ECO:0000250}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 98..101; /note=PTAP/PSAP motif; MOTIF 109..112; /note=PPXY motif |
| Gene Encoded By | |
| Mass | 64,421 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |