| IED ID | IndEnz0002011755 |
| Enzyme Type ID | protease011755 |
| Protein Name |
Alpha-lytic protease EC 3.4.21.12 Alpha-lytic endopeptidase |
| Gene Name | alpha-LP |
| Organism | Lysobacter enzymogenes |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Xanthomonadales Xanthomonadaceae Lysobacter Lysobacter enzymogenes |
| Enzyme Sequence | MYVSNHRSRRVARVSVSCLVAALAAMSCGAALAADQVDPQLKFAMQRDLGIFPTQLPQYLQTEKLARTQAAAIEREFGAQFAGSWIERNEDGSFKLVAATSGARKSSTLGGVEVRNVRYSLKQLQSAMEQLDAGANARVKGVSKPLDGVQSWYVDPRSNAVVVKVDDGATEAGVDFVALSGADSAQVRIESSPGKLQTTANIVGGIEYSINNASLCSVGFSVTRGATKGFVTAGHCGTVNATARIGGAVVGTFAARVFPGNDRAWVSLTSAQTLLPRVANGSSFVTVRGSTEAAVGAAVCRSGRTTGYQCGTITAKNVTANYAEGAVRGLTQGNACMGRGDSGGSWITSAGQAQGVMSGGNVQSNGNNCGIPASQRSSLFERLQPILSQYGLSLVTG |
| Enzyme Length | 397 |
| Uniprot Accession Number | P00778 |
| Absorption | |
| Active Site | ACT_SITE 235; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 262; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 342; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential cleavage: Ala-|-Xaa, Val-|-Xaa in bacterial cell walls, elastin and other proteins.; EC=3.4.21.12; |
| DNA Binding | |
| EC Number | 3.4.21.12 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (26); Chain (1); Disulfide bond (3); Helix (10); Propeptide (1); Sequence conflict (1); Signal peptide (1); Turn (3) |
| Keywords | 3D-structure;Direct protein sequencing;Disulfide bond;Hydrolase;Protease;Serine protease;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..24; /evidence=ECO:0000255 |
| Structure 3D | NMR spectroscopy (1); X-ray crystallography (48) |
| Cross Reference PDB | 1BOQ; 1GBA; 1GBB; 1GBC; 1GBD; 1GBE; 1GBF; 1GBH; 1GBI; 1GBJ; 1GBK; 1GBL; 1GBM; 1P01; 1P02; 1P03; 1P04; 1P05; 1P06; 1P09; 1P10; 1P11; 1P12; 1QQ4; 1QRW; 1QRX; 1SSX; 1TAL; 2ALP; 2H5C; 2H5D; 2LPR; 2PRO; 2ULL; 3LPR; 3M7T; 3M7U; 3PRO; 3QGJ; 3URC; 3URD; 3URE; 4PRO; 5LPR; 5WOT; 6LPR; 7LPR; 8LPR; 9LPR; |
| Mapped Pubmed ID | 15111063; 16834383; 1931963; 1998685; 22635267; 2611204; 2716847; 29374165; 3122831; 7500345; 9232638; |
| Motif | |
| Gene Encoded By | |
| Mass | 41,077 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.21.12; |