| IED ID | IndEnz0002011759 |
| Enzyme Type ID | protease011759 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase Usp2 EC 3.4.19.12 Ubiquitin specific protease 2 |
| Gene Name | Usp2 CG14619 |
| Organism | Drosophila melanogaster (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly) |
| Enzyme Sequence | MMLDIKKTRGFHKIPQASKLQSTTKTSSVVATSASSQNEVPSPGGSAGSKVGATNPVRAANQRFFLVSSYKDPTFLKAECDLAHAHVTTSKVKTTLQPHRRSRAGEDSRNNNYNTSRAPTLINMRRPSLFNGNQQPTTTNSTTINNTTSRNTTSNTSNGVLKYSVRSTTATATSTSTRNYGKLKPLNNNQTTAGVAMMNGHTNNNNNNTRNSSNINNGGNNNMQRQQQQHDDISFIDSDDPPATGGPEAGISTTKTSICYFKPITPPLQLRHEQNQVQQQEEQPQPSSSKSASHRYPRPKSTIIASAHSNFAASFEKFSGGLYRQTNGETNIESKTSSNARRYGIDSLSIKASIEKFNNLSGQKRQNPGSGSGIGPGSATASGLGGGRLSVASRSNHGSQAGGSSSNLQQRYSSDLDNIRVAAGYSSSLTRAAYRTTATMNSVSTPVAVTSELGGPISGDGGETATAMGQPTSKVTVRGAHSNRQPIECDSVVLTNKASKDATTAATPTVATATATHTPATSSVSTVTVTAAAPNSASDSTLARSGTGSSSTARSVLPPMTPTSSRYWDRDSGTSRSSIGTSSALNSSSLKHNSDDGYKTASSSRDEKSEGLCGLRNIGNTCFMNSVIQCLSHTQELTRFLRSHHGSRSLSTKDQQILHEFAKLIQEMWTANVHTVTPMELKRAFSTKHRMYSDYNQQDAQEFLRFFLDSLHSALNSGVKGETLNIDDNLSDNKKADLTWEWYTRHENSLVRDLFVGQLKSTLKCTTCGNTSVTFDPFWDLSVPLPSSSRCKLEACLDLFIREEVLDGDEMPTCAKCKTRRKCTKSFTIQRFPKYLVIHLKRFSETRWSKLSNIVEFPTSDSELNMGSYGANSNSNVHYSLYAISNHMGSTAGGHYVALCKHPVSRKWHEFNDNIVSDALSENHLVSSSAYILFYERT |
| Enzyme Length | 938 |
| Uniprot Accession Number | Q8IQ27 |
| Absorption | |
| Active Site | ACT_SITE 622; /note="Nucleophile"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01035, ECO:0000305|PubMed:25027767"; ACT_SITE 895; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01035" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000269|PubMed:25027767}; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Hydrolase that deubiquitinates polyubiquitinated target proteins (PubMed:25027767). Required for preventing the activation of the Toll signaling cascades under unchallenged conditions (PubMed:25027767). Essential for bodily calcium homeostasis (PubMed:26756164). {ECO:0000269|PubMed:25027767, ECO:0000269|PubMed:26756164}.; FUNCTION: [Isoform A]: Required for preventing the activation of the immune deficiency (Imd) signaling cascade under unchallenged conditions. Regulates the Imd pathway by specifically removing 'Lys-48'-linked ubiquitin from imd. Also promotes imd degradation probably by binding to imd and enhancing its association with the proteasome. {ECO:0000269|PubMed:25027767}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Alternative sequence (1); Chain (1); Compositional bias (6); Domain (1); Metal binding (4); Mutagenesis (1); Region (6) |
| Keywords | Alternative splicing;Hydrolase;Immunity;Innate immunity;Metal-binding;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11102377; 11744370; 20220848; 20371351; 20440302; 21074052; 22937016; 23071443; 23139745; 23275879; 23899565; 25242144; 25294943; 25312911; 25687947; 25848931; 26120032; 26870755; 28067623; 29050293; 30333215; 31722958; 32580388; 32656090; 33988679; |
| Motif | |
| Gene Encoded By | |
| Mass | 101,704 |
| Kinetics | |
| Metal Binding | METAL 765; /note=Zinc; /evidence=ECO:0000250|UniProtKB:O75604; METAL 768; /note=Zinc; /evidence=ECO:0000250|UniProtKB:O75604; METAL 814; /note=Zinc; /evidence=ECO:0000250|UniProtKB:O75604; METAL 817; /note=Zinc; /evidence=ECO:0000250|UniProtKB:O75604 |
| Rhea ID | |
| Cross Reference Brenda |