| IED ID | IndEnz0002011768 |
| Enzyme Type ID | protease011768 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase 30 EC 3.4.19.12 Deubiquitinating enzyme 30 Ubiquitin thioesterase 30 Ubiquitin-specific-processing protease 30 Ub-specific protease 30 |
| Gene Name | usp30 TEgg099b09.1 |
| Organism | Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amphibia Batrachia Anura Pipoidea Pipidae Xenopodinae Xenopus Silurana Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) |
| Enzyme Sequence | MSWAPVSTWSRRTPLAACCSAPELPPAGAWKACAAGSLRIGPQGRCKMMKNWGMIGGIAAALAAGIYVLWGPISDRKKYRKGLVPGLLNLGNTCFMNSLLQGLASCPSFIRWLADFTSKYRQENNTTEHQHLSVTLLHLLKALCNQEGTEDEVLDASPLLEVLRAHRWQISSFEEQDAHELFHVLTSSLEDERDRRPHVTHLFDLDSLEFPLEPQRQIHCRTQVPIYPIPSQWKSQHPFHGRLTSNMVCKHCQHQSPMRYDTFDSLSLSIPVATWGHPITLDQCLQHFISTESVKDVVCENCTKIHAAQIPNSQSVENRKTTFVKQLKLGKLPQCLCIHLQRLSWSNQGSPLKRNEHVQFSEFLAMDRFKYRISGCSTSKQPANHLSAAEQETTDGKEGGAQNPTMPFLNGACSTSYISPPFTSPLPTNPEWTSSSYLFRLMAVVVHHGDMHSGHFVTYRRSPAAKNQKLTSQQWLWISDDTVRRTNFQEVLSSSAYLLFYERIQSNLHHPEDQRAAEK |
| Enzyme Length | 519 |
| Uniprot Accession Number | A4QNN3 |
| Absorption | |
| Active Site | ACT_SITE 94; /note="Nucleophile"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093"; ACT_SITE 455; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000250|UniProtKB:Q70CQ3}; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Deubiquitinating enzyme that acts as a key inhibitor of mitophagy by counteracting the action of parkin (PRKN). {ECO:0000250|UniProtKB:Q70CQ3}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (1); Domain (1); Region (1); Sequence conflict (1); Topological domain (2); Transmembrane (1) |
| Keywords | Hydrolase;Membrane;Mitochondrion;Mitochondrion outer membrane;Protease;Reference proteome;Thiol protease;Transmembrane;Transmembrane helix;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Mitochondrion outer membrane {ECO:0000250|UniProtKB:Q70CQ3}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 58,683 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |