| IED ID | IndEnz0002011793 |
| Enzyme Type ID | protease011793 |
| Protein Name |
Tiggy-winkle hedgehog protein TWHH Sonic hedgehog protein B SHHB Cleaved into: Tiggy-winkle hedgehog protein N-product; Tiggy-winkle hedgehog protein C-product |
| Gene Name | shhb twhh |
| Organism | Danio rerio (Zebrafish) (Brachydanio rerio) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Actinopterygii Actinopteri Neopterygii Teleostei Osteoglossocephalai Clupeocephala Otomorpha Ostariophysi Otophysi Cypriniphysae Cypriniformes (carps and others) Cyprinoidei Danionidae Danioninae Danio Danio rerio (Zebrafish) (Brachydanio rerio) |
| Enzyme Sequence | MDVRLHLKQFALLCFISLLLTPCGLACGPGRGYGKRRHPKKLTPLAYKQFIPNVAEKTLGASGKYEGKITRNSERFKELIPNYNPDIIFKDEENTNADRLMTKRCKDKLNSLAISVMNHWPGVKLRVTEGWDEDGHHLEESLHYEGRAVDITTSDRDKSKYGMLSRLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGGCFPGSGTVTLGDGTRKPIKDLKVGDRVLAADEKGNVLISDFIMFIDHDPTTRRQFIVIETSEPFTKLTLTAAHLVFVGNSSAASGITATFASNVKPGDTVLVWEDTCESLKSVTVKRIYTEEHEGSFAPVTAHGTIIVDQVLASCYAVIENHKWAHWAFAPVRLCHKLMTWLFPARESNVNFQEDGIHWYSNMLFHIGSWLLDRDSFHPLGILHLS |
| Enzyme Length | 416 |
| Uniprot Accession Number | Q90419 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Intercellular signal essential for a variety of patterning events during development. Involved in dorso-ventral patterning of the brain and in early patterning of the developing eyes. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (3); Lipidation (2); Metal binding (12); Signal peptide (1); Site (3) |
| Keywords | Autocatalytic cleavage;Calcium;Cell membrane;Developmental protein;Hydrolase;Lipoprotein;Membrane;Metal-binding;Palmitate;Protease;Reference proteome;Secreted;Signal;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Tiggy-winkle hedgehog protein N-product]: Cell membrane {ECO:0000250}; Lipid-anchor {ECO:0000250}; Extracellular side {ECO:0000250}. Note=The N-terminal peptide remains associated with the cell surface. {ECO:0000250}.; SUBCELLULAR LOCATION: [Tiggy-winkle hedgehog protein C-product]: Secreted, extracellular space {ECO:0000250}. Note=The C-terminal peptide diffuses from the cell. {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | PTM: The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). This covalent modification appears to play an essential role in restricting the spatial distribution of the protein activity to the cell surface. The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity (By similarity). {ECO:0000250}. |
| Signal Peptide | SIGNAL 1..26; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11428131; 12588855; 15539490; 16049113; 16452095; 16481351; 16502420; 21118524; 22119054; |
| Motif | |
| Gene Encoded By | |
| Mass | 46,576 |
| Kinetics | |
| Metal Binding | METAL 92; /note=Calcium 1; /evidence=ECO:0000250|UniProtKB:Q15465; METAL 93; /note=Calcium 1; /evidence=ECO:0000250|UniProtKB:Q15465; METAL 93; /note=Calcium 2; /evidence=ECO:0000250|UniProtKB:Q15465; METAL 98; /note=Calcium 1; /evidence=ECO:0000250|UniProtKB:Q15465; METAL 128; /note=Calcium 1; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q15465; METAL 129; /note=Calcium 1; /evidence=ECO:0000250|UniProtKB:Q15465; METAL 129; /note=Calcium 2; /evidence=ECO:0000250|UniProtKB:Q15465; METAL 132; /note=Calcium 2; /evidence=ECO:0000250|UniProtKB:Q15465; METAL 134; /note=Calcium 2; /evidence=ECO:0000250|UniProtKB:Q15465; METAL 143; /note=Zinc; /evidence=ECO:0000250|UniProtKB:Q15465; METAL 150; /note=Zinc; /evidence=ECO:0000250|UniProtKB:Q15465; METAL 185; /note=Zinc; /evidence=ECO:0000250|UniProtKB:Q15465 |
| Rhea ID | |
| Cross Reference Brenda |