| IED ID | IndEnz0002011862 |
| Enzyme Type ID | protease011862 |
| Protein Name |
PspA protein 35 kDa protein |
| Gene Name | pspA Rv2744c MTV002.09c |
| Organism | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Corynebacteriales Mycobacteriaceae Mycobacterium Mycobacterium tuberculosis complex Mycobacterium tuberculosis Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
| Enzyme Sequence | MANPFVKAWKYLMALFSSKIDEHADPKVQIQQAIEEAQRTHQALTQQAAQVIGNQRQLEMRLNRQLADIEKLQVNVRQALTLADQATAAGDAAKATEYNNAAEAFAAQLVTAEQSVEDLKTLHDQALSAAAQAKKAVERNAMVLQQKIAERTKLLSQLEQAKMQEQVSASLRSMSELAAPGNTPSLDEVRDKIERRYANAIGSAELAESSVQGRMLEVEQAGIQMAGHSRLEQIRASMRGEALPAGGTTATPRPATETSGGAIAEQPYGQ |
| Enzyme Length | 270 |
| Uniprot Accession Number | P9WHP5 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Levels are regulated by proteolytic cleavage by PepD to help maintain cell envelope homeostasis. {ECO:0000269|PubMed:21445360, ECO:0000269|PubMed:25899163}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Involved in resistance to stress (PubMed:25899163, PubMed:27002134). Facilitates intracellular growth of M.tuberculosis (PubMed:25899163). Associates with and regulates lipid droplets (LDs) homeostasis under conditions of stress and may regulate non-replicating persistence (NRP) (PubMed:27002134). Could be involved in preservation of envelope integrity and tolerance to surface stress (PubMed:25899163). Has an inhibitory effect on ClgR activity (PubMed:25899163). May block ClgR activity after stress and then, form a multiprotein complex with Rv2743c-Rv2742c, leading to the release of ClgR (PubMed:25899163). {ECO:0000269|PubMed:25899163, ECO:0000269|PubMed:27002134}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Region (1) |
| Keywords | Cytoplasm;Reference proteome;Stress response;Virulence |
| Interact With | |
| Induction | INDUCTION: Expression is regulated by ClgR (PubMed:25899163). Induced by carbonyl cyanide m-chlorophenyl hydrazone (CCCP) (PubMed:25899163). Induced by the cell envelope stressor SDS (PubMed:27002134). {ECO:0000269|PubMed:25899163, ECO:0000269|PubMed:27002134}. |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:27002134}. Note=Localizes to the surface of lipid droplets (LDs). {ECO:0000269|PubMed:27002134}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 29,258 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |