| IED ID | IndEnz0002011900 |
| Enzyme Type ID | protease011900 |
| Protein Name |
Secreted aspartic protease LUC8 EC 3.4.23.- Lucilactaene biosynthesis cluster protein 8 |
| Gene Name | LUC8 |
| Organism | Fusarium sp. |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Hypocreomycetidae Hypocreales Nectriaceae Fusarium unclassified Fusarium Fusarium sp. |
| Enzyme Sequence | MMHAFHHLAVLLIGSLPASASTLPHPDGYRHVNGSCSKSVAVPAVWNGFGYLFNITVGTPPQELTMLSDWTWMSLFVRSGRCLNQYDPSLCLGTSGQTWFDERASTSFANTSLPQLSWPLTAFAPNFTVDYGTDDVCIGDLCSAGTVLQVSDFPYPGEGIPKVPFSGIFGMAPVTAGLNETFHPANYQAWKAGRLGSRVGWNSCAALASSDPCLGGEAKLVFGGTDSSLYDDDTLRIYEIQNPDWLSDAFYPLTPPRENYWTTPLTGSWILGTSEEESRNFAVPFSGSNGSNVTPLAVLDEGSEGLGAPLSLNAYNWLVDQVRGTLASNDTIEEIHAQGSSGFNTAEQNWYTVSCDDIDSYPELVYELNGHTNYTVPPQDYVTKLSDSSTCYLNINLWKYGRTEDGNAKVALLGLAFLKRLYVVLDFETQSFGLAPLSM |
| Enzyme Length | 439 |
| Uniprot Accession Number | A0A6J4B484 |
| Absorption | |
| Active Site | ACT_SITE 69; /evidence=ECO:0000255|PROSITE-ProRule:PRU01103; ACT_SITE 300; /evidence=ECO:0000255|PROSITE-ProRule:PRU01103 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.23.- |
| Enzyme Function | FUNCTION: Secreted aspartic protease; part of the gene cluster that mediates the biosynthesis of the mycotoxin lucilactaene and the lucilactaene-related compound NG-391 that act as cell cycle inhibitors with potent growth inhibitory activity against malarial parasites, moderate growth inhibitory activity against cancer cells, and no activity against bacteria and fungi (PubMed:32043422). Within the cluster, LUC5, LUC6, LUC2 and LUC1 are sufficient for lucilactaene production (Probable). The roles of the other LUC members are yet undetermined (Probable). {ECO:0000269|PubMed:32043422, ECO:0000305|PubMed:32043422}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Disulfide bond (1); Domain (1); Glycosylation (8); Signal peptide (1) |
| Keywords | Aspartyl protease;Disulfide bond;Glycoprotein;Hydrolase;Protease;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:D4ATH2}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..20; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 47,779 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |