| IED ID | IndEnz0002011906 |
| Enzyme Type ID | protease011906 |
| Protein Name |
Mitochondrial-processing peptidase subunit beta EC 3.4.24.64 Beta-MPP |
| Gene Name | mppB |
| Organism | Lentinula edodes (Shiitake mushroom) (Lentinus edodes) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Basidiomycota Agaricomycotina Agaricomycetes Agaricomycetidae Agaricales Omphalotaceae Lentinula Lentinula edodes (Shiitake mushroom) (Lentinus edodes) |
| Enzyme Sequence | MLGRVLKSAARSQRGLRSFATTTNLGPFTEISTLSNGLTVATESQPHAQTATVGVWIDAGSRAETDKTNGTAHFLEHMAFKGTGRRSQHALELEVENIGAHLNAYTSREQTVYYAKSFSKDVPVAVDIISDILQNSKLESGAIERERDVILREQQEVDKQLEEVVFDHLHAVAFQGQPLGRTILGPKNNILSIQRDDLASYIQTNYTADRMVLVGTGGVDHQSLVKLAEKHFSSLPVSANPLALGRLSSERKPTFVGSEARIRDDELPTAHVAIAVEGVGWSSPDYFPMMVMQSIFGNWDRSLGASSLLSSRLSHIISSNSLANSFMSFSTSYSDTGLWGIYLVSENLMNLDDTLHFTLKEWTRMSIAPTEGEVERAKSQLKAGLLLSLDGTTAVAEDIGRQIVTSGKRMTPAQIENAVDAVSVDDIKRVAQKYLWDKDFALAAFGNIDGLKDYGRIRNDMSSMLY |
| Enzyme Length | 466 |
| Uniprot Accession Number | Q9Y8B5 |
| Absorption | |
| Active Site | ACT_SITE 76; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10096 |
| Activity Regulation | ACTIVITY REGULATION: Binding to mppA is required for catalytic activity. {ECO:0000250|UniProtKB:P10507}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2.; EC=3.4.24.64; Evidence={ECO:0000250|UniProtKB:P10507}; |
| DNA Binding | |
| EC Number | 3.4.24.64 |
| Enzyme Function | FUNCTION: Catalytic subunit of the essential mitochondrial processing protease (MPP), which cleaves the mitochondrial sequence off newly imported precursors proteins (By similarity). Preferentially, cleaves after an arginine at position P2 (By similarity). {ECO:0000250|UniProtKB:P10507, ECO:0000250|UniProtKB:Q03346}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Metal binding (3); Transit peptide (1) |
| Keywords | Hydrolase;Metal-binding;Metalloprotease;Mitochondrion;Protease;Transit peptide;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000250|UniProtKB:P10507}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 51,163 |
| Kinetics | |
| Metal Binding | METAL 73; /note=Zinc; via tele nitrogen; /evidence=ECO:0000255|PROSITE-ProRule:PRU10096; METAL 77; /note=Zinc; via tele nitrogen; /evidence=ECO:0000255|PROSITE-ProRule:PRU10096; METAL 153; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU10096 |
| Rhea ID | |
| Cross Reference Brenda |