| IED ID | IndEnz0002011973 |
| Enzyme Type ID | protease011973 |
| Protein Name |
Metalloprotease YcaL EC 3.4.-.- |
| Gene Name | ycaL b0909 JW0892 |
| Organism | Escherichia coli (strain K12) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Escherichia coli (strain K12) |
| Enzyme Sequence | MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATLSDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSDVNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDAISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSFETLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK |
| Enzyme Length | 254 |
| Uniprot Accession Number | P43674 |
| Absorption | |
| Active Site | ACT_SITE 135; /evidence=ECO:0000250|UniProtKB:O75844 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.-.- |
| Enzyme Function | FUNCTION: Involved in the degradation of the LPS-assembly protein LptD. Degrades LptD that have engaged the Bam complex but are stalled at an early step in the outer membrane protein assembly process. {ECO:0000269|PubMed:28784813}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Compositional bias (1); Erroneous initiation (1); Lipidation (2); Metal binding (3); Region (1); Sequence conflict (1); Signal peptide (1) |
| Keywords | Cell inner membrane;Cell membrane;Hydrolase;Lipoprotein;Membrane;Metal-binding;Metalloprotease;Palmitate;Protease;Reference proteome;Signal;Zinc |
| Interact With | P0AAM7 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Lipid-anchor {ECO:0000255|PROSITE-ProRule:PRU00303}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255|PROSITE-ProRule:PRU00303 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 24561554; |
| Motif | |
| Gene Encoded By | |
| Mass | 26,740 |
| Kinetics | |
| Metal Binding | METAL 134; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:O75844; METAL 138; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:O75844; METAL 193; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:O75844 |
| Rhea ID | |
| Cross Reference Brenda |