| IED ID | IndEnz0002012016 |
| Enzyme Type ID | protease012016 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase 47 EC 3.4.19.12 Deubiquitinating enzyme 47 Ubiquitin thioesterase 47 Ubiquitin-specific-processing protease 47 |
| Gene Name | USP47 |
| Organism | Gallus gallus (Chicken) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Archelosauria Archosauria Dinosauria Saurischia Theropoda Coelurosauria Aves Neognathae Galloanserae Galliformes Phasianidae (turkeys) Phasianinae Gallus Gallus gallus (Chicken) |
| Enzyme Sequence | MKEFVSMRLLPEDMFWSCRQSTLAEMKKKFAQVESAAEEPRVLCIIQDTTNSKTVNERVTLNVPASTPLKKLFEDVASKVGYVNGTFDLVWGNGDNVTDMTPIDQNSDKTILDAGFEPGKKNFLHLTDKDGEQPHIMQEESGTTEDSAQDRFIGPLPREGSVGCTNDYVSQSYSYSSVLSKSETGYVGLVNQAMTCYLNSLLQTLFMTPEFRNALYKWEFEESEEDPVTSIPYQLQRLFVLLQTSKKRAIETTDVTRSFGWDSSEAWQQHDVQELCRVMFDALEQKWKQTEQADLINQLYQGKLKDYVRCLECGYEGWRIDTYLDIPLVIRPYGSNQAFASVEEALHAFIQPEILDGPNQYFCERCKKKCDARKGLRFLHFPYLLTLQLKRFDFDYTTMHRIKLNDRMTFPEELDMSIFIDVEDEKSPQTESCTDSGAENEGSCHSDQMSNDFSNDDGVDEGICLESNSAAERIAKVGSEKNSLLYELFSVMVHSGSAAGGHYYACIKSFSDDQWYSFNDQHVSKITQEDIKKTYGGSSGSRGYYSSAFASSTNAYMLIYRLKDPARNAKFLESHEYPDHIKQLVQKERELEEQEKRQREIERNTCKIKLFCMHPTKQIMMENKLEVHKDRTLKEAVGIAYKLMDLEEAVPLDCCRLVKYDEFHDYLERSYEGEEDTPMGLLLGGVKSTYMFDLLLETRRPDQIFQCYKPGEVMVKVHVVDLKTESVAPPISVRAYLNQTVSEFKQLISKATHLPAETMRVVLERCYNDLRLLTVSSKTLKAEGFFRSNKVFIESSESLDRHVAYTDSHLWKLLDRHANTIRLYVSLPEQSPGSQFRRSIYQKPSGDLGNLDEACERVKGPAGNMKSVEAILEESTEKLKSLSLQQQQQEGDNGDSSKSTEASDFENIESPSNEIDSSASVENRELENQIQISDPENLQSEERSDSDVNNDRSTSSVDSDILSSSHSSDTLCNVDNAPIPLANGLDSHSITSSRRSKANQGKKETWDTAEEDSGTDSEYDESGKSRGETQYMYFKSEPYTADEGSGEGQKWLMVHVDKRITLSAFKQQLEPFVGVPSSHFKVFRVYASNQEFESVRLNETLSSFSDDNKITIRLGRALKKGEYRVKVYQLLVNEPEPCKFLLDAVFAKGMTVRQSKEELLPQLREQCGLDLTIDRFRLRKKTWKNPGTVFLDYHIYEEDINISSNWEVFLEILDGVEKMKSMSQLAVLSRRWRPSEMKLDSFQEVVLESSSVEELKEKLSELSGIPLENIEFAKGRGTFPCDISVLEIHQDLDWNPKVSTLNVWPLYICDDGAVIFYRDKTEELMELTDEQRNELMKKESSRLQKTGHRVTYSPRKEKALKIYLDGAPNKDLTQD |
| Enzyme Length | 1375 |
| Uniprot Accession Number | E1C1R4 |
| Absorption | |
| Active Site | ACT_SITE 196; /note="Nucleophile"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093"; ACT_SITE 502; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Ubiquitin-specific protease that specifically deubiquitinates monoubiquitinated DNA polymerase beta (POLB), stabilizing POLB thereby playing a role in base-excision repair (BER). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (3); Domain (1); Region (3) |
| Keywords | Cytoplasm;DNA damage;DNA repair;Hydrolase;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 31982568; |
| Motif | |
| Gene Encoded By | |
| Mass | 157,247 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |