| IED ID | IndEnz0002012017 |
| Enzyme Type ID | protease012017 |
| Protein Name |
Ubiquitin C-terminal hydrolase 22 EC 3.4.19.12 Deubiquitinating enzyme 22 AtUBP22 Ubiquitin thioesterase 22 Ubiquitin-specific-processing protease 22 |
| Gene Name | UBP22 At5g10790 T30N20_60 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MSARISFLKNPDPCNHLSDYKLRYGTDGYKSFNNLFRCFNDARIKIKLQGIDIPRCSYCSVYQKRLYICLICRSISCSSHILLHTQLNKGHDIAIDVERSELYCCACIDQVYDSEFDEVVVSKQLFGLGMSVKSGADVVAVRSNKKRRLDSQLIIGSNFLVSPRDRREKWTFPLGLRGLNNLGSTCFMNAVLQALVHAPPLRNFWLSGQHNRDLCPRRTMGLLCLPCDLDVIFSAMFSGDRTPYSPAHLLYSWWQHSTNLATYEQQDSHEFFISLLDRIHENEGKSKCLYQDNEECQCITHKAFSGLLRSDVTCTTCGSTSTTYDPFIDISLTLDSMNGFSPADCRKNRYSGGPSVNAIMPTLSGCLDFFTRSEKLGPDQKLNCQSCGEKRESSKQMSIRRLPLLLCLHVKRFEHSLTRKTSRKIDSYLQYPFRLNMSPYLSSSIIGKRFGNRIFAFDGEGEYDSSSSSSPSAEFEIFAVVTHKGMLESGHYVTYLRLKGLWYRCDDAWINEVEEEVVRGCECYMLFYAQETVIQKAHKELSYQVISMADAFPFADC |
| Enzyme Length | 557 |
| Uniprot Accession Number | Q9LEW0 |
| Absorption | |
| Active Site | ACT_SITE 186; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10092; ACT_SITE 491; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10092 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000305}; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Component of a deubiquitination module (DUB module) that specifically deubiquinates monoubiquinated histone H2B (H2Bub) (PubMed:29588169, PubMed:30192741). Does not seem to be a component of the TREX-2 complex (PubMed:29588169). Seems to act independently of the SAGA multiprotein complex (PubMed:30192741). The DUB module is responsible for the major H2Bub deubiquitinase activity in Arabidopsis (PubMed:30192741). {ECO:0000269|PubMed:29588169, ECO:0000269|PubMed:30192741}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Domain (1); Metal binding (8); Sequence conflict (3); Zinc finger (1) |
| Keywords | Hydrolase;Metal-binding;Nucleus;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway;Zinc;Zinc-finger |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Nucleus, nucleoplasm {ECO:0000269|PubMed:30192741}. Note=Displays a rather patchy distribution forming a punctuated pattern in the euchromatin (PubMed:30192741). Does not localize in the heterochromatic chromocenters or nucleolus (PubMed:30192741). {ECO:0000269|PubMed:30192741}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 15319477; 18485060; 18775970; 28695315; 32745625; |
| Motif | |
| Gene Encoded By | |
| Mass | 63,518 |
| Kinetics | |
| Metal Binding | METAL 56; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 59; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 69; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 72; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 77; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 80; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 84; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 91; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502 |
| Rhea ID | |
| Cross Reference Brenda |