Detail Information for IndEnz0002012020
IED ID IndEnz0002012020
Enzyme Type ID protease012020
Protein Name Ubiquitin carboxyl-terminal hydrolase 2
EC 3.4.19.12
Deubiquitinating enzyme 2
Ubiquitin thioesterase 2
Ubiquitin-specific-processing protease 2
Gene Name UBP2 KLLA0E02376g
Organism Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Kluyveromyces Kluyveromyces lactis (Yeast) (Candida sphaerica) Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Enzyme Sequence MMASQEPALELNGEQRDSVSETAEVLRSKSLESFSDLVDDGKTLLYGDISKSFPFKTCDRILDDIRISPWFLKKFGSSVMKQPMLQYSRERQQLQPWNLVHLIDQVNLRSRYDYDSMTCPGKNTISVMFALLVDPNFTPNDFDDIDKFPEYFFHLKITVKRRSYLENFNRHVGITHYHVLEPESLHPFDKRDIFIMEEKDCRLVDQSIFVSADTNKLILVEIIKPEFNSENLAEYRTAKIEERYKNACQEFDLLNPDDIPSQAECLKTLFMIFKNPLQRKSANSEFKIISRDSVALNSQINTDWLTTMFDFSLQKTAVEDNVQSGEEYKPPDLVDYITDFKVRGIREAYTRKSMEVVLIGKQSMLLENELGTEKKTVAKCFSNQHFSASHTWWFNILNHQHIEPFPYDINYHFINLSVAFKYIDKDIIKNYETQIALDQENISHYFDALQYVTNAKGSYQLIAYCGKQDVVGYEDLNNALQVFGLDPTDIDASLLDANTMIEYYNSHLLRSSDNQRKDLRNALRVLGKYLGSQKMLFLVEYEPYYNVQQAYTLLKVDETVDDDIIQTAYTINIADAPGLKKDYDRAIFTIALDRRSIFLLNVLTDECPEFAQYYNCTDISYDEALNIIEIDMNASDDVILEVFQKKWSHGIMTGPDYLLKLKMALQNIGFTRNSKLINHFLDTGIVDVSCLPVATWPAGINNVGNTCYLNSLLQFFFTIKPLRDFILNYDDDSAKLLDASEYHSRRRIGGREVSKQEELRSVQFVYHLRDLFNDMIHTNSRCVTPTKELVYLAFAPSNVEVEFGDDTVAQKELIDLTTDVVEDPTDTTRHLASCDDDIIMCQSPVALPEHKTSSEAGTQQYSVQVAKISADQLENTLEIGRQQDVTECIGNVLAQLEIASEPLSLEDDLEQNDLVKQLFYGRIKQDLIPVNDEASVRTKYERFLSLLVNTGDHPKDIYDALDFYFQNDYLNLEEYGDVKRTVSISELPAVLQIQIQRVYYDREKFMPFKSIEPLPFGETLYMDRYMATEDPKLLAEIQQNAELKQKLQDLKQRQRKLLSQNEIGLTLKSSLIETKKFLQSGTLKAHDIDADNIPSSIAYIDILINNIDEELKSLFNKITDLETTISQQFSEFKHIGYSLFAVFIHRGEASYGHYWVYIKDHTKNGIWRKYNDDSVTEVPQSEVFNFTEGNTATPYFLVYVREGQEQETIEPLKRILQQQE
Enzyme Length 1220
Uniprot Accession Number O42726
Absorption
Active Site ACT_SITE 707; /note="Nucleophile"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093"; ACT_SITE 1153; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093"
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12;
DNA Binding
EC Number 3.4.19.12
Enzyme Function FUNCTION: Has an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety in natural or engineered linear fusion proteins, irrespective of their size or the presence of an N-terminal extension to ubiquitin. {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Domain (1); Sequence conflict (2)
Keywords Hydrolase;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 141,120
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda