| IED ID | IndEnz0002012031 |
| Enzyme Type ID | protease012031 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase 3 EC 3.4.19.12 |
| Gene Name | UCH3 At4g17510 dl4790c |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MATASESSSSKRWLPLESNPDVMNQYLWGLGLAPDEAECNDVYGLDDELLEMVPKPVLAVLFLYPITKKSEEERIEQDKEIKEKVHSDKVYFMKQTVGNACGTIGLLHAIGNITSEIKLSDGSFLDRFFKSTANMTPMERAKFLENDSQIEDAHSVAVIAGDTPASEDADTHFICLACVEGELYELDGRKAGPISHGASSPATLLKDATKVIKKMIEKNPGSLNFNLIAISKRT |
| Enzyme Length | 234 |
| Uniprot Accession Number | Q8GWE1 |
| Absorption | |
| Active Site | ACT_SITE 101; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:P09936; ACT_SITE 172; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:P09936 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000305}; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Erroneous gene model prediction (2); Site (1) |
| Keywords | Hydrolase;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 18650403; 18775970; 28627464; 30160354; 31745110; |
| Motif | |
| Gene Encoded By | |
| Mass | 25,712 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |