| IED ID | IndEnz0002012064 |
| Enzyme Type ID | protease012064 |
| Protein Name |
Vacuolar-processing enzyme beta-isozyme EC 3.4.22.34 Asparaginyl endopeptidase beta-VPE Beta-VPE |
| Gene Name | bVPE At1g62710 F23N19.7 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MAKSCYFRPALLLLLVLLVHAESRGRFEPKILMPTEEANPADQDEDGVGTRWAVLVAGSSGYGNYRHQADVCHAYQILRKGGLKEENIVVLMYDDIANHPLNPRPGTLINHPDGDDVYAGVPKDYTGSSVTAANFYAVLLGDQKAVKGGSGKVIASKPNDHIFVYYADHGGPGVLGMPNTPHIYAADFIETLKKKHASGTYKEMVIYVEACESGSIFEGIMPKDLNIYVTTASNAQESSYGTYCPGMNPSPPSEYITCLGDLYSVAWMEDSETHNLKKETIKQQYHTVKMRTSNYNTYSGGSHVMEYGNNSIKSEKLYLYQGFDPATVNLPLNELPVKSKIGVVNQRDADLLFLWHMYRTSEDGSRKKDDTLKELTETTRHRKHLDASVELIATILFGPTMNVLNLVREPGLPLVDDWECLKSMVRVFEEHCGSLTQYGMKHMRAFANVCNNGVSKELMEEASTAACGGYSEARYTVHPSILGYSA |
| Enzyme Length | 486 |
| Uniprot Accession Number | Q39044 |
| Absorption | |
| Active Site | ACT_SITE 169; /evidence=ECO:0000255; ACT_SITE 211; /evidence=ECO:0000255 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins and small molecule substrates at -Asn-|-Xaa- bonds.; EC=3.4.22.34; Evidence={ECO:0000250|UniProtKB:Q84LM2}; |
| DNA Binding | |
| EC Number | 3.4.22.34 |
| Enzyme Function | FUNCTION: Asparagine-specific endopeptidase involved in the processing of vacuolar seed protein precursors into the mature forms (By similarity). Probably involved in post-translational proteolysis of seed storage proteins in the protein storage vacuole of developing seeds (PubMed:12417707, PubMed:14688293). {ECO:0000250|UniProtKB:P49043, ECO:0000269|PubMed:12417707, ECO:0000269|PubMed:14688293}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (12); Chain (1); Erroneous gene model prediction (1); Glycosylation (1); Helix (20); Sequence conflict (4); Signal peptide (1); Site (1); Turn (5) |
| Keywords | 3D-structure;Glycoprotein;Hydrolase;Protease;Reference proteome;Signal;Thiol protease;Vacuole |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Vacuole {ECO:0000269|PubMed:7579169}. Protein storage vacuole {ECO:0000269|PubMed:10417725}. |
| Modified Residue | |
| Post Translational Modification | PTM: Auto-catalytic activation. {ECO:0000250|UniProtKB:Q84LM2}. |
| Signal Peptide | SIGNAL 1..21; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 6YSA; |
| Mapped Pubmed ID | 11351099; 18296723; 19154227; 20736450; 22822203; 25960260; 26048883; 31858133; 32719006; 8589932; |
| Motif | |
| Gene Encoded By | |
| Mass | 53,828 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |