| IED ID | IndEnz0002012100 |
| Enzyme Type ID | protease012100 |
| Protein Name |
Genome polyprotein Includes: Aspartic protease PR EC 3.4.23.- ; Reverse transcriptase RT EC 2.7.7.49 EC 2.7.7.7 |
| Gene Name | |
| Organism | Petunia vein clearing virus (isolate Shepherd) (PVCV) |
| Taxonomic Lineage | Viruses Riboviria Pararnavirae Artverviricota Revtraviricetes Ortervirales Caulimoviridae Petuvirus Petunia vein clearing virus Petunia vein clearing virus (isolate Shepherd) (PVCV) |
| Enzyme Sequence | MTSPSDYQSNSSLATTYSNAPKLSKALSNKYDYLYEVDILKENQKISDTYLPLLNPYSAFAKRSVTPWSQIRSLVQSKPRHVKEYVAASKLDQHPVFATGEEQFVTLHIPEEFASHWKSHQFTHIHFGAVKIALTYHGRKGQPVVARLALLDTRYLEYQHANLGTAEITLNAGTVFITLFPNFTMSLSDANLSTALKIQVQIQGAPLTKDSIQATLHYQIAWRVQNHAMDLTLPGGEEALFLKIDAGSGATQCTQVPRQLSKEDLIKILPDSWVTNYEKLREPEEPLRSTEVSMSKRHDKSVAISFDHSHYKKLRNTHHFMGMISDDVIVLDDPETFSKTLPSLMQTHDWIHHFQLDGRAVSWYKDPFDGHCPWDIDCQCYSCLYSEDEEDFEDGFPTKYKGIPRPGSIAERKMQEEANLKKLYEEKDPFVGSLSRPGKYEYLVRYDAPSWAKDPHLTVEPTGWDSDEPIPPKQPFTTRNTLPKIYMFNPLNYENNFPPLSSFSKDGADHTPKIPKRNVVLPSGAKDPTGDLEATVNWQTENALAQNRMLTTIDRTLKETVTKVDRVTDQSSKNQGLIKVLEQQLQDLNKRICPPGTSLFHFFDQQKSEMASLKEQIRLLKEQPQKNETDTPSYQSSYQPFHSFSSPYMPSNPPNSPFTTFANTPQPQPSLFSQYPIQPKSPNTFDLAKLVWEKKDAIAAEKRAKKKLQKDEVKQKTSLPPESKRPDPQSSSHLGDQFMISDPALPKVYALNEPSVPSEDTSSQSYISTEESVEDTDSFSVVSEESTQLSQLSSSSNDSPENNENTLPQTFMVRPTEPEISEVEDEVDGMTEEPIPERRPEITPPKMVGTGFHTFSLDDISITKWPERIQDFHTWMLTKQLVEREPFLILSEFTARLSGTLREWWNSVGPDDKNRFLTSQDFTWNIRILYSYFCGDQSQNKEELRRQIFEMKCLSYDRKKIDRHFQRMIKLFYHIGGDISLKQAFISSLPPILSERISALIKERGTSVTQMHVGDIRQTAFYVLDDLCSKRKFFNQMKKMSRDLEKACTKSDLIIKGDKGCSGYCNPSRRRKYKRFKLPSFKERDGRQYRKRRRFFRRSKTSKAMRQKPRSCFTCGKIGHFSRNCPQNKKSIKLISEIQKYTGIDIEDDLESVFSIEDEPSEDTLFSLEFYEEYAGEQYQITSYEAPKTENPPLPKIHTIVEIPQTEVKIYTSKWDKPISVIAFYDTGAAYSIMDPAILPSEYWIPHFRHFGTADDGILTTTVKTKHPITIEFFPGFKYTTKLLGSDIPGKDLLIGFDIYRQLNNKLRIGADGIRWKNQFKRYTEIPRLFQLTTSNELQQLEDVIKNQLCADSHVDFLSKCSHPLWLNQDFFIQLPFKKNENINPTKASHSGMNPEHLQLAIKECDELQQFDLIEPSDSQWACEAFYVNKRSEQVRGKLRLVINYQPLNHFLQDDKFPIPNKLTLFSHLSKAKLFSKFDLKSGFWQLGIHPNERPKTGFCIPDRHFQWKVMPFGLKTAPSLFQKAMIKIFQPILFSALVYIDDILLFSETLEDHIKLLNQFISLVKKFGVMLSAKKMILAQNKIQFLGMDFADGTFSPAGHISLELQKFPDTNLSVKQIQQFLGIVNYIRDFIPEVTEHISPLSDMLKKKPPAWGKCQDNAVKQLKQLAQQVKSLHIPSEGKKILQTDASDQYWSAVLLEEHNGKRKICGFASGKFKVSEQHYHSTFKEILAVKNGIKKFNFFLIHTNFLVEMDMRAFPKMIRLNPKIVPNSQLLRWAQWFSPYQFEVKHLKGKDNILADFLSRPHEFSQRLKNSPKVLMFQRRTRSSSTKSKADSSQSTGSSYKLSHNLPENPPEVFNLDYPWDTSVFLERRTFYELQVFKKYGGSILRPFGVDPEYPFAHIFIPNPTDFSEDLLWMFWYLLNHFHILMKFRCSKFSKIDQVNPWMLKFLLWFNNHNYWASLFKCMKGIKKYVVIWFYRPVNYYQGKLCALPHSSIVKWNHVSVLNDEDEYSELQRFIFQENKCIPKEIWPGSSGSWNYGNSDHPHGQWIRDALREYREMNDYFQDAQDPYPAYSKVDLTQEELNTLRITRSYGSSSEDADMVKRSIYTVQSNIVKDSPRKRKGKAKSRSSTRSEKRRAKNKCKYRSLHGEDWWIELGYSTKPSTPSWTQDSSSEPCV |
| Enzyme Length | 2179 |
| Uniprot Accession Number | Q91DM0 |
| Absorption | |
| Active Site | ACT_SITE 1226; /note=For protease activity; shared with dimeric partner; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339, Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560, ChEBI:CHEBI:173112; EC=2.7.7.49; Evidence={ECO:0000255|PROSITE-ProRule:PRU00405}; CATALYTIC ACTIVITY: Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339, Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560, ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|PROSITE-ProRule:PRU00405}; |
| DNA Binding | |
| EC Number | 3.4.23.-; 2.7.7.49; 2.7.7.7 |
| Enzyme Function | FUNCTION: Encodes presumably for at least four polypeptides: Movement protein (MP), capsid protein (CP), Protease (PR), and reverse transcriptase (RT). {ECO:0000305}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Compositional bias (6); Domain (1); Metal binding (3); Region (7); Zinc finger (1) |
| Keywords | Aspartyl protease;Coiled coil;DNA-binding;Endonuclease;Hydrolase;Magnesium;Metal-binding;Nuclease;Nucleotidyltransferase;Protease;RNA-directed DNA polymerase;Reference proteome;Transferase;Transport;Viral movement protein;Zinc;Zinc-finger |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 252,214 |
| Kinetics | |
| Metal Binding | METAL 1479; /note=Magnesium; catalytic; for reverse transcriptase activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU00405; METAL 1542; /note=Magnesium; catalytic; for reverse transcriptase activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU00405; METAL 1543; /note=Magnesium; catalytic; for reverse transcriptase activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU00405 |
| Rhea ID | RHEA:22508 |
| Cross Reference Brenda |