| IED ID | IndEnz0002012114 |
| Enzyme Type ID | protease012114 |
| Protein Name |
Thrombin-like enzyme kangshuanmei SVTLE EC 3.4.21.- Fibrinogen-clotting enzyme Snake venom serine protease SVSP |
| Gene Name | |
| Organism | Gloydius brevicaudus (Korean slamosa snake) (Agkistrodon halys brevicaudus) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Lepidosauria (lepidosaurs) Squamata (squamates) Bifurcata (split-tongued squamates) Unidentata Episquamata Toxicofera Serpentes (snakes) Colubroidea Viperidae Crotalinae (pit vipers) Gloydius Gloydius brevicaudus (Korean slamosa snake) (Agkistrodon halys brevicaudus) |
| Enzyme Sequence | VIGGDECNINEHRFLVALYHSRSRTFLCGGTLINQEWVLTAAHCDRFFMYIRLGMHNKNVNFDDEQRRSPKEKYFFRCSNNFTKWDKDIMLIRLDSPVNNSAHIAPLSLPSNPPSVGSVCRVMGWGQTTSPQEDLSDVPRCANINLFNFTVCRAAYPWLPATSRVLCAGDMEGGIDTCNRDSGGPLICNGQFQGIVSKGQNLCAQPRKPALYTKVFDHLDWIQSIIAGNKTVTCPP |
| Enzyme Length | 236 |
| Uniprot Accession Number | P85109 |
| Absorption | |
| Active Site | ACT_SITE 43; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P33589; ACT_SITE 88; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P33589; ACT_SITE 182; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P33589 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by 4-(2-aminoethyl)-benzensulfonyl fluoride. Not inhibited by antithrombin-III. {ECO:0000269|PubMed:11491462}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Thrombin-like snake venom serine protease. Cleaves bonds after Arg and Lys, converts fibrinogen (FGA and FGB) to fibrin and releases both fibrinopeptides A and B, and fibrinogen peptide Bbeta1-42. Has a blood clotting activity. {ECO:0000269|PubMed:11491462, ECO:0000269|PubMed:16908038}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (6); Domain (1); Glycosylation (4) |
| Keywords | Blood coagulation cascade activating toxin;Direct protein sequencing;Disulfide bond;Glycoprotein;Hemostasis impairing toxin;Hydrolase;Protease;Secreted;Serine protease;Toxin |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11491462, ECO:0000269|PubMed:16908038}. |
| Modified Residue | |
| Post Translational Modification | PTM: N-glycosylated by units composed of Fuc, Man, GlcNAc, Gal and NeuAC residues. {ECO:0000269|PubMed:16908038}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 26,432 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |