| IED ID | IndEnz0002012140 |
| Enzyme Type ID | protease012140 |
| Protein Name |
Mitochondrial inner membrane i-AAA protease supercomplex subunit YME1 EC 3.4.24.- Protein OSD1 Tat-binding homolog 11 Yeast mitochondrial escape protein 1 |
| Gene Name | YME1 OSD1 YTA11 YPR024W YP9367.04 |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Enzyme Sequence | MNVSKILVSPTVTTNVLRIFAPRLPQIGASLLVQKKWALRSKKFYRFYSEKNSGEMPPKKEADSSGKASNKSTISSIDNSQPPPPSNTNDKTKQANVAVSHAMLATREQEANKDLTSPDAQAAFYKLLLQSNYPQYVVSRFETPGIASSPECMELYMEALQRIGRHSEADAVRQNLLTASSAGAVNPSLASSSSNQSGYHGNFPSMYSPLYGSRKEPLHVVVSESTFTVVSRWVKWLLVFGILTYSFSEGFKYITENTTLLKSSEVADKSVDVAKTNVKFDDVCGCDEARAELEEIVDFLKDPTKYESLGGKLPKGVLLTGPPGTGKTLLARATAGEAGVDFFFMSGSEFDEVYVGVGAKRIRDLFAQARSRAPAIIFIDELDAIGGKRNPKDQAYAKQTLNQLLVELDGFSQTSGIIIIGATNFPEALDKALTRPGRFDKVVNVDLPDVRGRADILKHHMKKITLADNVDPTIIARGTPGLSGAELANLVNQAAVYACQKNAVSVDMSHFEWAKDKILMGAERKTMVLTDAARKATAFHEAGHAIMAKYTNGATPLYKATILPRGRALGITFQLPEMDKVDITKRECQARLDVCMGGKIAEELIYGKDNTTSGCGSDLQSATGTARAMVTQYGMSDDVGPVNLSENWESWSNKIRDIADNEVIELLKDSEERARRLLTKKNVELHRLAQGLIEYETLDAHEIEQVCKGEKLDKLKTSTNTVVEGPDSDERKDIGDDKPKIPTMLNA |
| Enzyme Length | 747 |
| Uniprot Accession Number | P32795 |
| Absorption | |
| Active Site | ACT_SITE 541; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: Catalytic subunit of the mitochondrial inner membrane i-AAA protease supercomplex required for mitochondrial inner membrane protein turnover. The protease is probably ATP-dependent. Important to maintain the integrity of the mitochondrial compartment. Required both for the degradation of unassembled subunit 2 of cytochrome c oxidase (COX2) and for efficient assembly of mitochondrial respiratory chain. Binds unfolded substrates in an ATPase-independent manner; binding of folded COX2, a physiological substrate, requires an active ATPase but when COX2 is destabilized an active ATPase is no longer necessary. {ECO:0000269|PubMed:16267274, ECO:0000269|PubMed:16527490}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 321..328; /note=ATP; /evidence=ECO:0000255 |
| Features | Active site (1); Chain (1); Compositional bias (3); Helix (5); Metal binding (3); Mutagenesis (7); Nucleotide binding (1); Region (2); Sequence conflict (3) |
| Keywords | 3D-structure;ATP-binding;Hydrolase;Membrane;Metal-binding;Metalloprotease;Mitochondrion;Mitochondrion inner membrane;Nucleotide-binding;Protease;Reference proteome;Zinc |
| Interact With | P25573 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:8355690, ECO:0000269|PubMed:8688560}; Peripheral membrane protein {ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:8355690, ECO:0000269|PubMed:8688560}; Matrix side {ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:8355690, ECO:0000269|PubMed:8688560}. Note=Although this protein does not have any predicted transmembrane helices it behaves like an integral membrane protein. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | NMR spectroscopy (1) |
| Cross Reference PDB | 2MV3; |
| Mapped Pubmed ID | 10192337; 10453730; 10494627; 10782097; 10882099; 10906275; 10930580; 10996302; 11212342; 11553723; 11737636; 11830665; 11996120; 12172961; 12191771; 12524335; 14690591; 15772085; 16429126; 16725216; 16966379; 17261594; 17492370; 17893242; 17976194; 18298044; 19289147; 19307606; 19536198; 19930686; 20398622; 20629757; 20657548; 20675578; 21300850; 21439406; 21463454; 21926328; 22001671; 22094424; 22292080; 22498346; 22589700; 22663076; 22687516; 22783546; 22808036; 22808334; 22993211; 23036860; 23479443; 23508107; 23910823; 24025448; 24201294; 24648523; 25573113; 25576874; 25688091; 25833713; 25840011; 26182355; 26774473; 27036363; 27068958; 27068967; 27398316; 27488107; 27693354; 29097521; 29138251; 7803857; 8514129; 8861950; 9149530; 9635427; 9683639; 9695811; 9857067; 9891785; |
| Motif | |
| Gene Encoded By | |
| Mass | 81,772 |
| Kinetics | |
| Metal Binding | METAL 540; /note=Zinc; catalytic; /evidence=ECO:0000250; METAL 544; /note=Zinc; catalytic; /evidence=ECO:0000250; METAL 618; /note=Zinc; catalytic; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda | 3.4.24.B19; |