| IED ID | IndEnz0002012143 |
| Enzyme Type ID | protease012143 |
| Protein Name |
Zinc metalloproteinase-disintegrin-like AAV1 EC 3.4.24.- Snake venom metalloproteinase SVMP Cleaved into: Disintegrin-like AAV1-C Fragment |
| Gene Name | |
| Organism | Deinagkistrodon acutus (Hundred-pace snake) (Agkistrodon acutus) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Lepidosauria (lepidosaurs) Squamata (squamates) Bifurcata (split-tongued squamates) Unidentata Episquamata Toxicofera Serpentes (snakes) Colubroidea Viperidae Crotalinae (pit vipers) Deinagkistrodon Deinagkistrodon acutus (Hundred-pace snake) (Agkistrodon acutus) |
| Enzyme Sequence | DVVSPPVCGN |
| Enzyme Length | 10 |
| Uniprot Accession Number | P0DJH3 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: The proteinase activity is slightly enhanced by Ca(2+) and Mg(2+). Inhibited by EDTA and phenanthroline, but not by PMSF. {ECO:0000269|PubMed:17029743}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: Zinc metalloprotease-disintegrin AAV1: snake venom zinc metalloprotease that binds to the platelet collagen receptor glycoprotein VI (GP6) thus inhibiting collagen- and convulxin- (a GP6 agonist) induced platelet aggregation and the tyrosine phosphorylation of intracellular signaling proteins (LAT, SYK, p76SLP76, PIK3C, and PLCG2) that follows GP6 activation. Interestingly, blocks platelet aggregation but does not affect shape change. Also degrades the Aalpha chain of fibrinogen (FGA), followed by the Bbeta chain (FGB). {ECO:0000269|PubMed:17029743}.; FUNCTION: Disintegrin AAV1-C: 30 kDa fragment of AAV1 autoproteolysed in absence of Ca(2+) that shows more potent inhibition of convulxin-induced platelet aggregation than AAV1. {ECO:0000269|PubMed:17029743}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (2); Disulfide bond (1); Domain (1); Metal binding (2); Non-terminal residue (2) |
| Keywords | Calcium;Cell adhesion impairing toxin;Direct protein sequencing;Disulfide bond;Glycoprotein;Hemostasis impairing toxin;Hydrolase;Metal-binding;Metalloprotease;Platelet aggregation inhibiting toxin;Protease;Secreted;Toxin;Zinc;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | PTM: N-glycosylated. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 986 |
| Kinetics | |
| Metal Binding | METAL 7; /note=Calcium 1; via carbonyl oxygen; /evidence=ECO:0000250; METAL 10; /note=Calcium 1; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda |