| IED ID | IndEnz0002012144 |
| Enzyme Type ID | protease012144 |
| Protein Name |
Thrombin-like enzyme halystase SVTLE EC 3.4.21.- Snake venom serine protease SVSP |
| Gene Name | |
| Organism | Gloydius blomhoffii (Mamushi) (Agkistrodon halys blomhoffi) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Lepidosauria (lepidosaurs) Squamata (squamates) Bifurcata (split-tongued squamates) Unidentata Episquamata Toxicofera Serpentes (snakes) Colubroidea Viperidae Crotalinae (pit vipers) Gloydius Gloydius blomhoffii (Mamushi) (Agkistrodon halys blomhoffi) |
| Enzyme Sequence | IIGGDECNINEHRFLVALYTPRSRTLFCGGTLINQEWVLTAAHCDRKNFRIKLGMHSKKVPNKDEQTRVPKEKFFCLSSKNYTLWDKDIMLIRLDSPVKNSTHIEPFSLPSSPPSVGSVCRIMGWGRISPTEETFPDVPHCVNINLLEYEMCRAPYPEFELPATSRTLCAGILEGGKDTCRGDSGGPLICNGQFQGIASWGDDPCAQPHKPAAYTKVFDHLDWIKSIIAGNTDASCPP |
| Enzyme Length | 238 |
| Uniprot Accession Number | P81176 |
| Absorption | |
| Active Site | ACT_SITE 43; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 88; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 184; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by diisopropylfluorophosphate (DFP), PMSF and leupeptin. {ECO:0000269|PubMed:9546675}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Thrombin-like snake venom serine protease. Cleaves fibrinogen (beta chain of fibrinogen (FGB) and more slowly alpha chain (FGA)) without inducing fibrin clotting and cleaves kininogen to produce bradykinin (KNG), resulting in the reduction of blood pressure. {ECO:0000269|PubMed:9546675}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (6); Domain (1); Glycosylation (2); Natural variant (1) |
| Keywords | Direct protein sequencing;Disulfide bond;Fibrinogenolytic toxin;Glycoprotein;Hemostasis impairing toxin;Hydrolase;Hypotensive agent;Protease;Secreted;Serine protease;Toxin |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 26,483 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |