| IED ID | IndEnz0002012165 |
| Enzyme Type ID | protease012165 |
| Protein Name |
Zinc metalloproteinase/disintegrin Cleaved into: Snake venom metalloproteinase HR2a SVMP EC 3.4.24.53 Snake venom metalloproteinase HR2b Trimerelysin II ; Disintegrin flavostatin Platelet aggregation activation inhibitor |
| Gene Name | |
| Organism | Protobothrops flavoviridis (Habu) (Trimeresurus flavoviridis) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Lepidosauria (lepidosaurs) Squamata (squamates) Bifurcata (split-tongued squamates) Unidentata Episquamata Toxicofera Serpentes (snakes) Colubroidea Viperidae Crotalinae (pit vipers) Protobothrops Protobothrops flavoviridis (Habu) (Trimeresurus flavoviridis) |
| Enzyme Sequence | MIEVLLVTICLAVFPYPGSSIILESGNVDDYEVVYPQKLTALPKGAVQPKYEDAMQYEFKVNGEPVVLHLEKNKGLFSEDYSETHYSPDGREITTYPSVEDHCYYHGRIQNDADSTASISACDGLKGYFKLQGETYLIEPLELSDSEAHAVFKYENVEKEDEAPKMCGVTQNWESDESIKKASQLYLTPEQQRFPQRYIELAIVVDHGMYTKYSSNFKKIRKRVHQMVNNINEMYRPLNIAITLSLLDVWSEKDLITMQAVAPTTARLFGDWRETVLLKQKDHDHAQLLTDINFTGNTIGWAYMGGMCNAKNSVGIVKDHSSNVFMVAVTMTHEIGHNLGMEHDDKDKCKCEACIMSAVISDKPSKLFSDCSKDYYQTFLTNSKPQCIINAPLRTDTVSTPVSGNEFLEAGEECDCGSPSNPCCDAATCKLRPGAQCADGLCCDQCRFKKKRTICRRARGDNPDDRCTGQSADCPRNS |
| Enzyme Length | 478 |
| Uniprot Accession Number | P14530 |
| Absorption | |
| Active Site | ACT_SITE 334 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Cleavage of 3-Asn-|-Gln-4, 10-His-|-Leu-11 and 14-Ala-|-Leu-15 in the insulin B chain, and the bond Z-Gly-Pro-|-Leu-Gly-Pro in a small molecule substrate of microbial collagenase.; EC=3.4.24.53; |
| DNA Binding | |
| EC Number | 3.4.24.53 |
| Enzyme Function | FUNCTION: [Snake venom metalloproteinase HR2a]: Zinc protease that induces hemorrhage. {ECO:0000269|PubMed:9114455}.; FUNCTION: [Disintegrin flavostatin]: Inhibits platelet aggregation induced by ADP, thrombin, and collagen. Acts by inhibiting fibrinogen interaction with platelet receptors GPIIb/GPIIIa (ITGA2B/ITGB3). {ECO:0000269|PubMed:9114455}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (2); Disulfide bond (9); Domain (2); Metal binding (7); Modified residue (1); Motif (1); Natural variant (3); Propeptide (2); Region (1); Signal peptide (1); Site (1) |
| Keywords | Calcium;Cell adhesion impairing toxin;Direct protein sequencing;Disulfide bond;Glycoprotein;Hemorrhagic toxin;Hemostasis impairing toxin;Hydrolase;Metal-binding;Metalloprotease;Platelet aggregation inhibiting toxin;Protease;Pyrrolidone carboxylic acid;Secreted;Signal;Toxin;Zinc;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | MOD_RES 191; /note="Pyrrolidone carboxylic acid"; /evidence="ECO:0000269|PubMed:2753880, ECO:0000269|PubMed:7597726" |
| Post Translational Modification | PTM: Not N-glycosylated. {ECO:0000269|PubMed:2753880}. |
| Signal Peptide | SIGNAL 1..20; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 459..461; /note=Cell attachment site |
| Gene Encoded By | |
| Mass | 53,646 |
| Kinetics | |
| Metal Binding | METAL 200; /note=Calcium; /evidence=ECO:0000250; METAL 284; /note=Calcium; /evidence=ECO:0000250; METAL 333; /note=Zinc; catalytic; METAL 337; /note=Zinc; catalytic; METAL 343; /note=Zinc; catalytic; METAL 387; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000250; METAL 390; /note=Calcium; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda |