| IED ID | IndEnz0002012248 |
| Enzyme Type ID | protease012248 |
| Protein Name |
Polyprotein pp62 60 kDa polyprotein p60 62 kDa polyprotein p62 Cleaved into: p15 PIG1 ; p35; p8 |
| Gene Name | Mal-102 |
| Organism | African swine fever virus (isolate Tick/Malawi/Lil 20-1/1983) (ASFV) |
| Taxonomic Lineage | Viruses Varidnaviria Bamfordvirae Nucleocytoviricota Pokkesviricetes Asfuvirales Asfarviridae Asfivirus African swine fever virus (ASFV) African swine fever virus (isolate Tick/Malawi/Lil 20-1/1983) (ASFV) |
| Enzyme Sequence | MPSNMKQFCKISVWLQQHDPDLLEIINNLCMLGNLSAAKYKHGVTFIYPKQAKIRDEIKKHAYSNDPSQAIKTLESLILPFYIPTPAEFTGEIGSYTGVKLEVEKTEANKVILKNGEAVLIPAADFKPFPDRRLAVWIMESGSMPLEGPPYKRKKEGGGNDPPVPKHISPYTPRTRIAIEVEKAFDDCMRQNWCSVNNPYLAKSVSLLSFLSLNHPTEFIKVLPLIDFDPLVTFYLLLEPYKTHGDDFLIPETVLFGPTGWNGTDLYQSAMLEFKKFFTQITRQTFMDIADTATKEVDVPICYSDPETVHSYANHVRTEILHHNMVNKVTTPNLVVQAYNELEQTNTIRHYGPIFPESTINALRFWKKLWQDEQRFVIHGLHRTLMDQPTYETSEFAEIVRNLRFSRPGNNYINELNITSPAMYGDKHTTGDIAPNDRFAMLVAFINSTDFLYTAIPEEKVGGNDTQTSSLTDLVPTRLHSFLNHNLSKLKILNRAQQTVRNILSNDCLNQLKHYVKHTGKNEILKLLQD |
| Enzyme Length | 530 |
| Uniprot Accession Number | P0CA06 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: [Polyprotein pp62]: Essential for the correct assembly and maturation of the core of the virion. {ECO:0000250|UniProtKB:Q65179}.; FUNCTION: [p35]: Component of the core shell (By similarity). Binds to phosphatidylserine, which may enable the core shell binding with the inner membrane (By similarity). {ECO:0000250|UniProtKB:Q65179}.; FUNCTION: [p15]: Component of the core shell (By similarity). Binds to phosphatidylserine and DNA, which may link the core shell to the inner membrane and to the viral nucleoid (By similarity). {ECO:0000250|UniProtKB:Q65179}.; FUNCTION: [p8]: Component of the core shell. {ECO:0000250|UniProtKB:Q65179}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (4); Initiator methionine (1); Site (2) |
| Keywords | Disulfide bond;Host cytoplasm;Late protein;Ubl conjugation;Virion |
| Interact With | |
| Induction | INDUCTION: Expressed in the late phase of the viral replicative cycle. {ECO:0000250|UniProtKB:Q65179}. |
| Subcellular Location | SUBCELLULAR LOCATION: [Polyprotein pp62]: Host cytoplasm, host perinuclear region {ECO:0000250|UniProtKB:Q65179}. Note=Found in perinuclear cytoplasmic viral factories during assembly. {ECO:0000250|UniProtKB:Q65179}.; SUBCELLULAR LOCATION: [p35]: Virion {ECO:0000250|UniProtKB:Q65179}. Note=Located in the core shell, which functions like a matrix between the DNA and the inner envelope. {ECO:0000250|UniProtKB:Q65179}.; SUBCELLULAR LOCATION: [p15]: Virion {ECO:0000269|PubMed:7853518}. Note=Located in the core shell, which functions like a matrix between the DNA and the inner envelope. {ECO:0000250|UniProtKB:Q65179}.; SUBCELLULAR LOCATION: [p8]: Virion {ECO:0000250|UniProtKB:Q65179}. Note=Located in the core shell, which functions like a matrix between the DNA and the inner envelope. {ECO:0000250|UniProtKB:Q65179}. |
| Modified Residue | |
| Post Translational Modification | PTM: [p15]: Monoubiquitinated in vitro by viral UBCv1. {ECO:0000269|PubMed:7853518}.; PTM: [Polyprotein pp62]: Specific enzymatic cleavages in vivo yield mature proteins. {ECO:0000250|UniProtKB:Q65179}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 60,563 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |