| IED ID | IndEnz0002012270 |
| Enzyme Type ID | protease012270 |
| Protein Name |
Thrombin-like enzyme okinaxobin-2 SVTLE EC 3.4.21.- Fibrinogen-clotting enzyme Okinaxobin II Snake venom serine protease SVSP Fragment |
| Gene Name | |
| Organism | Ovophis okinavensis (Ryukyu Island pit viper) (Trimeresurus okinavensis) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Lepidosauria (lepidosaurs) Squamata (squamates) Bifurcata (split-tongued squamates) Unidentata Episquamata Toxicofera Serpentes (snakes) Colubroidea Viperidae Crotalinae (pit vipers) Ovophis Ovophis okinavensis (Ryukyu Island pit viper) (Trimeresurus okinavensis) |
| Enzyme Sequence | VVGGDECNINEHRFLVALYY |
| Enzyme Length | 20 |
| Uniprot Accession Number | P0C578 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Strongly inactivated by diisopropylfluorophosphate (DFP) and to a lesser extent by tosyl-L-lysine chloromethyl ketone (TLCK). {ECO:0000269|PubMed:7725319}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Thrombin-like snake venom serine protease. Releases both fibrinopeptides A and B from fibrinogen (FGA and FGB) to form fibrin clots. {ECO:0000269|PubMed:7725319}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 8.0. {ECO:0000269|PubMed:7725319}; |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Domain (1); Non-terminal residue (1) |
| Keywords | Blood coagulation cascade activating toxin;Direct protein sequencing;Glycoprotein;Hemostasis impairing toxin;Hydrolase;Protease;Secreted;Serine protease;Toxin |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:7725319}. |
| Modified Residue | |
| Post Translational Modification | PTM: Glycosylated. {ECO:0000269|PubMed:7725319}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 2,312 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=118 uM for tosyl-L-arginine methyl ester (TAME) {ECO:0000269|PubMed:7725319}; KM=67 uM for benzoyl-L-arginine p-nitroanilide (BAPA) {ECO:0000269|PubMed:7725319}; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |