| IED ID | IndEnz0002012288 |
| Enzyme Type ID | protease012288 |
| Protein Name |
Mitochondrial respiratory chain complexes assembly protein YTA12 EC 3.4.24.- Tat-binding homolog 12 |
| Gene Name | YTA12 RCA1 YMR089C YM9582.14C |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Enzyme Sequence | MLLLSWSRIATKVVRRPVRFRSYYGLTHIKSLHTQYRLLNRLQENKSGNKNEDNNEDAKLNKEIPTDEEVEAIRKQVEKYIEQTKNNTIPANWKEQKRKIDESIRRLEDAVLKQESNRIQEERKEKEEENGPSKAKSNRTKEQGYFEGNNSRNIPPPPPPPPPKPPLNDPSNPVSKNVNLFQIGLTFFLLSFLLDLLNSLEEQSEITWQDFREKLLAKGYVAKLIVVNKSMVKVMLNDNGKNQADNYGRNFYYFTIGSIDSFEHKLQKAQDELDIDKDFRIPVLYVQEGNWAKAMFQILPTVLMIAGIIWLTRRSAQAAGGSRGGIFGLSRSKAKKFNTETDVKIKFKDVAGCDEAKEEIMEFVSFLKEPSRYEKMGAKIPRGAILSGPPGTGKTLLAKATAGEAGVPFYFVSGSEFVEMFVGVGAARVRDLFKTARENAPSIVFIDEIDAIGKARQKGNFSGANDERENTLNQMLVEMDGFTPADHVVVLAGTNRPDILDKALLRPGRFDRHINIDKPELEGRKAIFAVHLHHLKLAGEIFDLKNRLAALTPGFSGADIANVCNEAALIAARSDEDAVKLNHFEQAIERVIGGVERKSKLLSPEEKKVVAYHEAGHAVCGWYLKYADPLLKVSIIPRGQGALGYAQYLPGDIFLLTEQQLKDRMTMSLGGRVSEELHFPSVTSGASDDFKKVTSMATAMVTELGMSDKIGWVNYQKRDDSDLTKPFSDETGDIIDSEVYRIVQECHDRCTKLLKEKAEDVEKIAQVLLKKEVLTREDMIDLLGKRPFPERNDAFDKYLNDYETEKIRKEEEKNEKRNEPKPSTN |
| Enzyme Length | 825 |
| Uniprot Accession Number | P40341 |
| Absorption | |
| Active Site | ACT_SITE 614; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: Acts as a component of the m-AAA protease complex which is a ATP-dependent metalloprotease mediating degradation of non-assembled mitochondrial inner membrane proteins. The complex is necessary for the assembly of mitochondrial respiratory chain and ATPase complexes. Function both in post-translational assembly and in the turnover of mistranslated or misfolded polypeptides. {ECO:0000269|PubMed:8681382, ECO:0000269|PubMed:9707443}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 388..395; /note=ATP; /evidence=ECO:0000255 |
| Features | Active site (1); Chain (1); Compositional bias (3); Metal binding (3); Mutagenesis (1); Nucleotide binding (1); Region (3); Sequence conflict (3); Transmembrane (2) |
| Keywords | ATP-binding;Hydrolase;Membrane;Metal-binding;Metalloprotease;Mitochondrion;Nucleotide-binding;Protease;Reference proteome;Transmembrane;Transmembrane helix;Zinc |
| Interact With | P39925 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000269|PubMed:8681382}; Multi-pass membrane protein {ECO:0000269|PubMed:8681382}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10207067; 10453730; 10782097; 10882099; 10906275; 10930580; 10996302; 11212342; 11996120; 12191771; 12417197; 12791261; 14623864; 15205678; 16239145; 16429126; 16554755; 16647881; 16725216; 16778770; 17245427; 17615298; 19019989; 19221197; 19453275; 19536198; 19748349; 19748354; 19841731; 20208537; 21147776; 21300850; 21439406; 21447998; 21610694; 22001671; 22022284; 22172993; 22253597; 22498346; 22663076; 22910375; 23479443; 24393166; 25927548; 26030272; 26035862; 27693354; 29030426; 8810243; 8988248; 9106654; 9149530; 9342402; 9635427; 9857067; |
| Motif | |
| Gene Encoded By | |
| Mass | 93,276 |
| Kinetics | |
| Metal Binding | METAL 613; /note=Zinc; catalytic; /evidence=ECO:0000250; METAL 617; /note=Zinc; catalytic; /evidence=ECO:0000250; METAL 689; /note=Zinc; catalytic; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda | 3.4.24.B18; |