| IED ID | IndEnz0002012292 |
| Enzyme Type ID | protease012292 |
| Protein Name |
Trypsin-1 EC 3.4.21.4 Trypsin I |
| Gene Name | |
| Organism | Astacus astacus (Noble crayfish) (Astacus fluviatilis) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Crustacea Multicrustacea Malacostraca Eumalacostraca Eucarida Decapoda Pleocyemata Astacidea (true lobsters and crayfishes) Astacoidea (crayfish) Astacidae Astacus Astacus astacus (Noble crayfish) (Astacus fluviatilis) |
| Enzyme Sequence | IVGGTDAVLGEFPYQLSFQETFLGFSFHFCGASIYNENYAITAGHCVYGDDYENPSGLQIVAGELDMSVNEGSEQTITVSKIILHENFDYDLLDNDISLLKLSGSLTFNNNVAPIALPAQGHTATGNVIVTGWGTTSEGGNTPDVLQKVTVPLVSDAECRDDYGADEIFDSMICAGVPEGGKDSCQGDSGGPLAASDTGSTYLAGIVSWGYGCARPGYPGVYTEVSYHVDWIKANAV |
| Enzyme Length | 237 |
| Uniprot Accession Number | P00765 |
| Absorption | |
| Active Site | ACT_SITE 45; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 96; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 189; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.; EC=3.4.21.4; |
| DNA Binding | |
| EC Number | 3.4.21.4 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (3); Domain (1); Metal binding (3); Site (1) |
| Keywords | Calcium;Digestion;Direct protein sequencing;Disulfide bond;Hydrolase;Metal-binding;Protease;Secreted;Serine protease |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, extracellular space. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 25,022 |
| Kinetics | |
| Metal Binding | METAL 64; /note=Calcium; /evidence=ECO:0000250; METAL 69; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000250; METAL 74; /note=Calcium; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda |