| IED ID | IndEnz0002012317 |
| Enzyme Type ID | protease012317 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase 22 EC 3.4.19.12 Deubiquitinating enzyme 22 Ubiquitin thioesterase 22 Ubiquitin-specific-processing protease 22 |
| Gene Name | USP22 KIAA1063 USP3L |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MVSRPEPEGEAMDAELAVAPPGCSHLGSFKVDNWKQNLRAIYQCFVWSGTAEARKRKAKSCICHVCGVHLNRLHSCLYCVFFGCFTKKHIHEHAKAKRHNLAIDLMYGGIYCFLCQDYIYDKDMEIIAKEEQRKAWKMQGVGEKFSTWEPTKRELELLKHNPKRRKITSNCTIGLRGLINLGNTCFMNCIVQALTHTPLLRDFFLSDRHRCEMQSPSSCLVCEMSSLFQEFYSGHRSPHIPYKLLHLVWTHARHLAGYEQQDAHEFLIAALDVLHRHCKGDDNGKKANNPNHCNCIIDQIFTGGLQSDVTCQVCHGVSTTIDPFWDISLDLPGSSTPFWPLSPGSEGNVVNGESHVSGTTTLTDCLRRFTRPEHLGSSAKIKCSGCHSYQESTKQLTMKKLPIVACFHLKRFEHSAKLRRKITTYVSFPLELDMTPFMASSKESRMNGQYQQPTDSLNNDNKYSLFAVVNHQGTLESGHYTSFIRQHKDQWFKCDDAIITKASIKDVLDSEGYLLFYHKQFLEYE |
| Enzyme Length | 525 |
| Uniprot Accession Number | Q9UPT9 |
| Absorption | |
| Active Site | ACT_SITE 185; /note="Nucleophile"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093"; ACT_SITE 479; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000269|PubMed:18206973}; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation and cell cycle progression. {ECO:0000269|PubMed:18206972, ECO:0000269|PubMed:18206973, ECO:0000269|PubMed:18469533}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Alternative sequence (1); Chain (1); Domain (1); Erroneous initiation (1); Frameshift (2); Metal binding (12); Modified residue (1); Mutagenesis (2); Sequence conflict (3); Zinc finger (1) |
| Keywords | Acetylation;Activator;Alternative splicing;Cell cycle;Chromatin regulator;Hydrolase;Metal-binding;Nucleus;Protease;Reference proteome;Thiol protease;Transcription;Transcription regulation;Ubl conjugation pathway;Zinc;Zinc-finger |
| Interact With | Q9NRN7; Q96AE4; Q13469; Q9NRN7; Q14CW9; O95429; Q5JR59-3 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q5DU02}. |
| Modified Residue | MOD_RES 129; /note=N6-acetyllysine; /evidence=ECO:0007744|PubMed:19608861 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 15931389; 16169070; 17980597; 19615732; 20824490; 21039844; 21337558; 21691749; 21735131; 21746879; 21773699; 21779003; 21900206; 21928107; 22067483; 22447106; 22880026; 23217440; 23300749; 23361242; 23382074; 23412977; 23437046; 23664741; 23979981; 24466336; 24561192; 24573640; 24802393; 24969755; 24993031; 25070659; 25111486; 25241842; 25241857; 25323692; 25369910; 25416956; 25445209; 25482932; 25546086; 25547493; 25817787; 25902005; 25907317; 25909224; 25971547; 26143114; 26195632; 26497847; 26638075; 27030811; 27057639; 27501329; 27983930; 28160502; 28415621; 28417539; 28427243; 28682440; 29174979; 29689565; 29720480; 29788550; 29981430; 30223389; 30224477; 30338802; 30338942; 30543303; 30598339; 30837379; 31527800; 31587155; 31685642; 31689236; 31740444; 31801945; 31842906; 32130408; 32216173; 32294625; 32494025; 32665011; 32687947; 32857323; 32896826; 33090397; 33369872; 33475455; 33826947; 33884267; 34007022; 34155658; 34226501; 34339800; 34396448; 34954996; |
| Motif | |
| Gene Encoded By | |
| Mass | 59,961 |
| Kinetics | |
| Metal Binding | METAL 23; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 25; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 63; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 66; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 76; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 79; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 84; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 89; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 93; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 99; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 112; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 115; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502 |
| Rhea ID | |
| Cross Reference Brenda |